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AI and Flow Cytometry Workshop

Conceptual illustration of cells, a flow cytometry stream, automation and collaboration with logos for NIST and NIAID underneath.
Credit: Rachel Trello, NIST, NIAID/NIH

A NIST–FDA–NIAID Co-Organized Workshop

This workshop aims to advance AI/ML applications in flow cytometry and related data. The workshop will focus on overcoming challenges and identifying solutions including essential measurements, reference controls, AI-ready reference data and ML/AI models. These efforts will target emerging applications in disease diagnosis, therapeutic development, manufacturing, and clinical trials as well as dosing and monitoring.

Draft agenda as of April 9, 2025

Day and Time

Session

Monday, June 9, 2025 

10:30 am – 10:45 am EDT
7:30 am – 7:45 am PDT

Welcome

10:45 am – 11:45 am EDT
7:45 am – 8:45 am PDT

Setting the stage 

Max Qian, PhD, J. Craig Venter Institute
The evolution of computational cytometry:  milestones, challenges and opportunities. 

Thomas Liechti, Roche and ISAC
Immunology with High-Dimensional Flow Cytometry

12:00 pm – 1:00 pm EDT
9:00 am – 10:00 am PDT

Break

1:00 pm – 3:00 pm EDT
10:00 am – 12:00 pm PDT

Flow cytometry in research and the clinic – Part I 

John Quinn, BD Life Sciences, Data Driven Insights and Future Trends
Guang Fan, Oregon Health & Science University, Title TBA 
Yu-Fen (Andrea) Wang, AHEAD Medicine, Title TBA
Kamila Czechowska-Kusio, Metafora Biosystems, Title TBA
Speaker TBA, National Cancer Institute, Title, TBA
Panel Discussion 

3:00 pm – 3:30 pm EDT
12:00 pm – 12:30 pm PDT

Break

3:30 pm – 5:00 pm EDT
12:30 pm – 2:00 pm PDT

Challenges and potential solutions for AI/ML applications – Part I 

Holden Maecker, Stanford University, Featured Presentation, Methods for producing high-quality clinical flow data
Ryan Brinkman, Dotmatics, Research and Clinical Data and the SOULCAP framework
Santosh Putta, Revvity, Learning cell types across diverse flow cytometry data sets
Xing Qiu, University of Rochester, Integrating flow cytometry and bulk transcriptomics for cell type-specific gene expression analysis

Tuesday, June 10, 2025 

10:30 am – 10:45 am EDT
7:30 am – 7:45 am PDT

Welcome, Day 2

10:45 am – 12:00 pm EDT
7:45 am – 9:00 am PDT

Flow Data Repositories and AI Resources

•  Anupama Gururaj, NIAID/NIH, NIAID ImmPort
• Jonathan Irish, Univ. Colorado and ISAC, FlowRepository by ISAC
• Varun Chandola, National Science Foundation, National AI Resources Overview

12:00 pm – 1:00 pm EDT
9:00 am – 10:00 am PDT

Break

1:00 pm – 3:00 pm EDT
10:00 am – 12:00 pm PDT

Updates on NIST FCSC Interlaboratory Studies and Centralized Data Analysis

• Lili Wang, NIST, Assay Interlaboratory Study Overview
John Elliott, NIST, Review of Data from Interlaboratory Studies and Data Repository LabCAS
Santosh Putta, Revvity, Centralized Data Analyses from Interlaboratory Studies
• Panel Discussion

3:00 pm – 3:30 pm EDT
12:00 pm – 12:30 pm PDT

Break

3:30 pm – 4:30 pm EDT
12:30 pm – 1:30 pm PDT

Advanced Technologies Showcase Presentations

• Curiox
• Cellarcus
• LumaCyte
• ThinkCyte
• NIST, Standards for Cell Therapy Characterization
• NIST, Instrument Interlaboratory Study Overview
• NIST, Gene Delivery Systems Interlaboratory Study Overview

4:30 pm – 4:45 pm EDT
1:30 pm – 1:45 pm PDT
Conclusion
Created November 6, 2024, Updated April 9, 2025