My research mostly focuses on using quantitative and standardized flow cytometry to measure clinically and biomanufacturing relevant biological substances, cells, extracellular vesicles/exosomes, virus, proteins, DNA and RNA. Current effort under the flow cytometry standards consortium includes: 1) development of additional reference fluorophore solutions, methods, and data for standardizing flow cytometer intensity scale and performance characteristics in the unit of the equivalent number of reference fluorophores (ERF) through ERF measurement service; 2) development of biological reference standards, control materials, and best practice procedures and methods for standardizing flow cytometric assays in counting of bio-entities and analysis of biomarker expression levels; 3) building infrastructure for flow cytometry data repository and multi-modal simultaneous data analysis for reference data generation. In addition to the consortium activities, current projects include: 1) characterization of biological reference materials including cell lines, engineered cell lines, primary cells, lyophilized cells, synthetic cell mimics, viral vectors, and exosomes; 2) quantifying expression of cell surface and intracellular biomarkers by estimating the antibodies bound per cell (ABC); 3) enumeration of cells with specific phenotypic characteristics and function, e.g. CD34+ stem cells and cytokine producing T cells; 4) harmonizing flow cytometry data across different cytometer platforms through panel design, antibody titration, instrument calibration, reference cell controls, and data analysis.