Unexpectedly high encapsulation efficiency in nanometer scale liposomes with hydrodynamic focusing using continuous-flow microfluidcs

Published

October 20, 2008

Author(s)

Andreas Jahn

Abstract

A method is presented for achieving high entrapment efficiencies of a hydrophilic drug simulant, sulforhodamine B (SRB), in nanometer-scale liposomes using a continuous-flow microfluidic system. Liposome size and size dispersion are determined using tandem Asymmetric Flow Field Flow Fractionation (AF4) and Multi-Angle Laser Light Scattering (MALLS). The average number of encapsulated SRB molecules in a single liposome is measured with Fluorescence Correlation Spectroscopy (FCS) and Fluorescence Cumulant Analyis (FCA). Our results show that this system allows for controlled loading of SRB into the liposomes with high entrapment efficiencies (EE) measured as a ratio of SRB concentration inside the liposome to SRB starting concentration in the hydration buffer.

Proceedings Title

Proceedings of the 12 International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2008)

Conference Dates

October 12-16, 2008

Conference Location

San Diego, CA

Pub Type

Conferences

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Keywords

liposomes, encapsulation, microfluidics, viscosity anisotropy

Citation

Jahn, A. (2008), Unexpectedly high encapsulation efficiency in nanometer scale liposomes with hydrodynamic focusing using continuous-flow microfluidcs, Proceedings of the 12 International Conference on Miniaturized Systems for Chemistry and Life Sciences (microTAS 2008), San Diego, CA, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=33074 (Accessed January 2, 2025)