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Search Publications by: Lili Wang (Fed)

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Displaying 1 - 25 of 157

Standards and Metrology for Viral Vectors as Molecular Tools: Outcomes from a CCQM Workshop

May 24, 2024
Author(s)
Janathan Campbell, Neil Almond, Y Bae, Ravneet Bhuller, Andrea Briones, S-Y CHO, Megan Cleveland, Thomas Cleveland, Francis Galaway, Hua-Jun He, U Herbrand, Jim Huggett, Sarah Kempster, Ibolya Kepiro, Afifa Khan, Edward Kwee, Wilson Li, Sheng Lin-Gibson, Luise Luckau, Caterina Minelli, M Ryadnov, I Searing, Lili Wang, Alexandra Whale, Julian Braybrook
Viral vectors are agents enabling gene transfer and genome editing and have widespread utility across the healthcare and biotechnology sectors. In January 2023, the International Bureau for Weights and Measures' Consultative Committee for Amount of

Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19

April 1, 2024
Author(s)
Jerilyn Izac, Edward Kwee, Adolfas Gaigalas, Lili Wang
COVID-19 is a global pandemic caused by the highly infectious SARS-CoV-2 virus. Efforts to combat SARS-CoV-2 infection include mass vaccination and development of monoclonal and convalescent plasma therapeutics that require precise measurements of

Standardization of Flow Cytometric Detection of Antigen Expression

January 25, 2024
Author(s)
Linhua Tian, Aaron Nelson, Tyler Lowe, Linda Weaver, Constance Yuan, Paul C. DeRose, Maryalice Stetler-Stevenson, Lili Wang
Background: While response to antigen-based immunotherapy relies upon the level of antigen expression by tumor cells and as decreasing levels of antigen expression can be an early indicator of developing resistance to therapy, we have developed an antigen

Monoclonal Antibodies as SARS-CoV-2 Serology Standards: Experimental Validation and Broader Implications for Correlates of Protection

October 28, 2023
Author(s)
Lili Wang, Paul Patrone, Anthony Kearsley, Jerilyn Izac, Adolfas Gaigalas, John Prostko, Hang Xie, Linhua Tian, Elzafir Elsheikh, Edward Kwee, Troy Kemp, Simon Jochum, Natalie Thornburg, Clifford McDonald, Adi Gundlapalli, Sheng Lin-Gibson
COVID-19 has highlighted the need for more accurate and reproducible measurements of humoral immunity, including antibody levels and neutralization potential, which are critical for diagnostics, seroprevalence estimates, and development of vaccines and

Size and Number Concentration Measurements of Polystyrene Nanoparticle Suspensions using Several Different Techniques

September 8, 2022
Author(s)
Paul C. DeRose, Kurt D. Benkstein, Elzafir B. Elsheikh, Adolfas K. Gaigalas, Sean Lehman, Dean C. Ripple, Linhua Tian, Wyatt N. Vreeland, Adam York, Yu-Zhong Zhang, Lili Wang
The number concentrations of nominal 100 nm, 200 nm and 500 nm diameter, fluorescently-labeled polystyrene nanosphere suspensions were measured using seven different techniques. Diameter values were also measured where possible. The diameter values were

CD19 Expression Analysis Using Two Flow cytometric Quantitative Schemes: An approach for Identifying Robust B-cell Reference Control Materials

March 19, 2021
Author(s)
Hao-Wei Wang, Paul Patrone, Anthony Kearsley, Jessica Watson, Liang Lim, Jothir Pichaandi, Olga Ornatsky, Daniel Majonis, Rukmini Bhardwaj, Howard Mostowski, Steven Bauer, Heba Degheidy, Lili Wang
Cell-based therapies have emerged as a novel approach to treat cancer and other conditions. Quantitative flow cytometry plays an increasingly important role in predicting therapy outcome in a wide range of hematologic malignancies. Several studies

Measurement of serological and neutralizing antibodies specific to SARS-CoV-2 virus in humans with COVID-19 disease

March 8, 2021
Author(s)
Linhua Tian, Elzafir B. Elsheikh, Paul Patrone, Anthony Kearsley, adolfas Gaigalas, Sarah L. Inwood, Sheng Lin-Gibson, Dominic Esposito, Lili Wang
The global response to COVID-19 has led to rapid advances in diagnostic, surveillance, and vaccine development. Critical measurements underpinning many of these efforts include serological assays to assess seroprevalence of populations and the complex

Introduction and Clinical Validation of Metrology Standards for Immunohistochemistry

December 21, 2020
Author(s)
Emina E. Torlakovic, Seshi Sompuram, Vani Kodela, Anika Schaedle, Paul DeRose, Steve Bogen, Lili Wang
Despite the widespread integration of immunohistochemistry (IHC) into routine surgical pathology, its use suffers from significant inter-laboratory disparities. The root cause and an unsolved technical challenge is the lack of metrology standards, the

Towards defining reference materials for extracellular vesicle size, concentration, refractive index and epitope abundance

September 24, 2020
Author(s)
Joshua Welsh, Edwin van der Pol, Britta Bettin, David Carter, An Hendrix, Metka Lenassi, Marc-Andre Langlois, Alicia Liorente, Arthur van de Nes, Rienk Nieuwland, Vera Tang, Kenneth Witwer, Jennifer Jones, Lili Wang
Accurate characterization of extracellular vesicles (EVs) is critical to explore their diagnostic and therapeutic applications. As the EV research field has developed, so too have the techniques used to characterize them. The development of reference

Expanding NIST’s Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles

September 16, 2020
Author(s)
Paul C. DeRose, Linhua Tian, Elzafir B. Elsheikh, Aaron A. Urbas, Yu-Zhong Zhang, Lili Wang
NIST, NIH and other industry stakeholders have been working together to enable fluorescence intensities of flow cytometer calibration beads to be assigned quantitative 'ERF' values with high accuracy and precision. The ultimate goal of this effort is to

Measurement and Standardization Challenges for Exosome-Based Delivery Vectors

September 4, 2020
Author(s)
Bryant C. Nelson, Lili Wang, Samantha D. Maragh, Paul C. DeRose, Elzafir B. Elsheikh, Wyatt N. Vreeland, Ionita Ghiran, Jennifer Jones
Extracellular vesicles (EVs), and in particular exosomes, have the potential to revolutionize the development and efficient delivery of clinical therapeutics. In this Perspective, we focus on providing a brief introduction to the landscape of exosome-based

An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes

September 3, 2020
Author(s)
Deepa Rajagopal, Linhua Tian, Shiqiu Xiong, Lili Wang, Jonathan Campbell, Luisa Saraiva, S Vessillier
Activation of T lymphocytes leads to differentiation, characterized by the ability to induce production of cytokines. Accurate determination of cellular subsets that secrete particular cytokine(s) is therefore, a significant parameter for functional

Workshop 13: Building Measurement Assurance in Flow Cytometry

June 17, 2019
Author(s)
Lili Wang, Stephen Perfetto, Robert Hoffman, John T. Elliott, Sheng Lin-Gibson, Steven Bauer, Heba Degheidy, Judith Arcidiacono, Litwin Virginia
Two workshops were held to identify measurement challenges and potential solutions for building measurement assurance for flow cytometry. This report summarizes key findings, including the need for high quality reagents, reference standards or materials

Workshop 9: Control Cells or Not

June 17, 2019
Author(s)
Paul Wallace, Jonni S. Moore, Derek Jones, Litwin Virginia, Lili Wang, Yanli Liu
"Control Cells or Not" was an educational workshop that used surveys, lectures, and discussions to identify problems and solutions related to using commercially available control cell products and lab-developed approaches. Collective efforts are proposed