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Patty Kiesler (Fed)
Biologist
Patty is a Biologist in the Biomarker and Genomic Sciences Group. Her work focuses on developing standardized measurements, protocols and benchmark datasets for the molecular characterization of biotechnology products created using genome editing technologies. She also contributes to the efforts of the NIST Genome Editing Consortium to implement measurement assurance to the evaluation of genome editing outcomes and to the advancement of projects in the NIST Genome Editing Program.
Prior to joining NIST, Patty worked in the field of DNA diagnostics performing clinical analysis of variants detected in genetic testing and other related tasks including creating and revising workflows, training resources and Standard Operating Procedures (SOPs). As a graduate student and post-doctoral fellow, Patty’s research focused on the functional characterization of variants associated with allergic phenotypes and genes associated with intestinal inflammation.
Research interests
- Genome editing measurement assurance
- CRISPR/Cas editing systems
- End-to-end genome editing workflows
- Off-target genome editing approaches
- Detection and quantification of genome editing outcomes
CURRENT PROJECTS
NIST Genome Editing Consortium
NIST Genome Editing Consortium: Working Group 1 - Specificity Measurements
PAST PUBLICATIONS
Bai R, Balog A, Kiesler P, Arjona D, Cui H. Comment on the Criteria for Interpretation of Mitochondrial tRNA Variants. Genetics in Medicine, 2020, 22 (18): 1418-1419
Jones K, Bryant S. Luo J, Kiesler P, Koontz S, Warrn J, Malech H, Kang E, and Dveksler G. Recombinant Pregnancy-Specific Glycoprotein 1 Has a Protective Role in a Murine Model of Acute Graft-versus-Host Disease. Biology of Blood and Marrow Transplantation, 2019, 25 (2): 193-203
Kiesler P, Fuss IJ, and Strober W. Experimental Models of Inflammatory Bowel Disease. CMGH Cellular and Molecular Gastroenterology and Hepatology, 2015, 1 (2): 154-170
Park Y, Choi S, Kiesler P, Gil-Krzewska A, Borrego F, Weck J, Krzewski K, and Coligan JE. Complex Regulation of Human NKG2D-DAP10 Cell Surface Expression: Opposing Roles of the γc cytokines and TGF-β1. Blood, 2011, 118 (11): 3019-3027
Kiesler P, Haynes PA, Shi L, Kao PN, Wysocki VH, and Vercelli D. NF90 and NF45 Regulate HS4-Dependent Interleukin-13 (IL13) Transcription in T Cells. The Journal of Biological Chemistry, 2010, 285 (11): 8256-8267
Kiesler P, Shakya A, Tantin D, and Vercelli D. An Allergy-Associated Polymorphism in a Novel Regulatory Element Enhances IL13 Expression. Human Molecular Genetics, 2009, 18 (23): 4513-4520
Cameron L, Webster RB, Strempel JM, Kiesler P, Kabesch M, Ramachandran H, Yu L, Stern DA, Graves PE, Lohman IC, Wright AL, Halonen M, KlimeckiWT, and Vercelli D. Th2 Cell-Selective Enhancement of Human IL13 Transcription by IL-13-1112C>T, a Polymorphism Associated with Allergic Inflammation. The Journal of Immunology, 2006, 177 (12): 8633-8642