Establishing Traceability to NIST SRM 2391c: PCR-Based DNA Profiling Standard

Published

September 16, 2015

Author(s)

Carolyn R. Steffen, Margaret C. Kline, David L. Duewer, Peter Vallone

Abstract

The NIST Standard Reference Material (SRM) 2391c: PCR-Based DNA Profiling Standard was updated in April 2015 to contain new information relevant to the forensic community. Previously, there were certified genotypes for 24 autosomal STR markers plus Amelogenin and 17 Y-STR markers. Due to the increase in markers present in larger commercial autosomal STR and Y-STR multiplex kits recently released, there is a need to add certified types for these new markers for each component of SRM 2391c (Components A-F). The updated Certificate of Analysis has certified values for 1 additional autosomal STR marker (D6S1043) and 12 additional Y-STR markers (29 total). Also, the number of STR multiplex assays tested increased from 24 to 43. Sanger sequencing was performed on Components A-C, E and F (Component D is a mixture of Components A and C) for all the certified markers to determine the STR repeat motifs and to characterize adjacent flanking regions and underlying polymorphisms (sequence, insertion-deletion, variation in complex motifs) typically not detected by fragment-based typing. SRM 2391c Components A-F can be used to establish traceability in a laboratory based on the updated certified and reference genotypes/haplotypes (information values cannot be used for NIST traceable materials). The simple steps to achieve NIST traceability with SRM 2391c will be explained.

Citation

Forensic Science International: Genetics Supplement Series

Volume

5

Pub Type

Journals

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DOI Link

Keywords

DNA sequencing, standard reference material, short tandem repeat, DNA typing, Sanger sequencing, traceable material

Citation

Steffen, C. , Kline, M. , Duewer, D. and Vallone, P. (2015), Establishing Traceability to NIST SRM 2391c: PCR-Based DNA Profiling Standard, Forensic Science International: Genetics Supplement Series, [online], https://doi.org/10.1016/j.fsigss.2015.09.045 (Accessed July 17, 2024)