Mechanisms of Osteoblast Adhesion on 3D Polymer Scaffolds Made by Rapid Prototyping

Published

April 1, 2005

Author(s)

T DuttaRoy, Francis W. Wang, J J. Stone, E H. Cho, S J. Lockett

Abstract

In this study, we looked at the adhesion of osteoblasts on 3D polycaprolactone (PCL) scaffolds compared to 2D PCL films and glass coverlsips. This adhesion was quantified after 24 hours by staining for vinculin, a marker of cell-substrate adhesion, and viewing the cells with confocal laser scanning microscopy (CLSM). Cells adhered on the glass and PCL films, with a larger number of cell spread area and number of focal adhesions on the glass compared to the PCL films (p<.05). Cells on the PCL scaffolds had a smaller cell spread area and lower number of focal adhesions compared to both glass and PCL films (p<.05). However, large clusters of cells were evident on the struts of the PCL scaffolds, indicating that cells did adhere, but that the mechanism of adhesion in 3D may differ from that in 2D.

Proceedings Title

Abstract for Society for Biomaterials| 30th Annual Meeting and Exposition

Volume

37

Conference Dates

April 27-30, 2005

Conference Location

Undefined

Conference Title

Abstract for Society for Biomaterials

Pub Type

Conferences

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Keywords

biomaterials, cell adhesion, cell culture, rapid prototyping, scaffold

Citation

DuttaRoy, T. , Wang, F. , Stone, J. , Cho, E. and Lockett, S. (2005), Mechanisms of Osteoblast Adhesion on 3D Polymer Scaffolds Made by Rapid Prototyping, Abstract for Society for Biomaterials| 30th Annual Meeting and Exposition, Undefined, [online], https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=852850 (Accessed November 8, 2024)