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Displaying 351 - 375 of 1174

Integrative and comparative reproductive biology: From alligators to xenobiotics

November 1, 2016
Author(s)
Ashley S. Boggs-Russell, John Bowden, Abby G. Wenzel, Satomi Kohno, Heather J. Hamlin, Lori Cruze, Krista A. McCoy, Alison M. Roark, Thea M. Edwards, Theresa M. Cantu, Jessica A. Cloy-McCoy, Nicole A. McNabb, Cameron E. Williams
Dr. Louis J. Guillette Jr. thought of himself as a reproductive biologist. However, his interest in reproductive biology transcended organ systems, life history stages, species, and environmental contexts. His integrative and collaborative nature led to

Enhanced Sensitivity of Neil1-/- Mice to Chronic UVB Exposure

October 28, 2016
Author(s)
Pawel Jaruga, M Miral Dizdar, Marcus J. Calkins, Vladimir Vartanian, Amanda McCullough, R. S. Lloyd, Guldal Kirkali
Oxidative stress and oxidatively induced DNA base damage are central mediators of UV-induced carcinogenesis and skin aging. However, increased steady-state levels of oxidatively induced DNA base damage have not been reported after chronic UV exposure

Toward Achieving Harmonization in a Nano-cytotoxicity Assay Measurement through an Interlaboratory Comparison Study

September 29, 2016
Author(s)
John T. Elliott, Matthias Roesslein, Nam W. Song, Blaza Toman, Agnieszka Kinsner-Ovaskainen, Rawiwan Maniratanachote, Marc L. Salit, Elijah J. Petersen, Fatima Sequeira, Jieun Lee, Francois Rossi, Cordula Hirsch, Harald Krug, Wongsakorn Suchaoin, Peter Wick
Design and development of reliable cell-based nanotoxicology assays is important for ranking of potential hazardous engineered nanomaterials. Challenges to producing a reliable assay protocol include working with nanoparticle dispersions and living cell

Elevated urinary levels of 8-hydroxy-2’-deoxyguanosine, (5’R)- and (5’S)-8,5’-cyclo-2’- deoxyadenosines, and 8-iso-prostaglandin F2? as potential biomarkers of oxidative stress in patients with prediabetes

September 26, 2016
Author(s)
M Miral Dizdar, Melis Kant, Merve Akis, Mehmet Calan, Tugba Arkan, Firat Bayraktar, Huray Islekel
Prediabetes is the preclinical stage of type 2 diabetes mellitus with intermediate state of hyperglycemia. Hyperglycemia results in a state of oxidative stress and that reactive oxygen species contribute to the production of insulin resistance, β-cell

After oxidation, zinc nanoparticles lose their ability to enhance responses to odorants

September 20, 2016
Author(s)
Samantha Hagerty, Yasmine C. Daniels, Melissa Singletary, Oleg Pustovyy, Ludmila Globa, William A. MacCrehan, Shinichiro Muramoto, June W. Lau, Edward Morrison, Iryna Sorokulova, Vitaly Vodyanoy, Gheorghe Stan
Electrical responses of olfactory sensory neurons to odorants were examined in the presence of zinc nanoparticles of various sizes and degrees of oxidation. The zinc nanoparticles were prepared by the underwater electrical discharge method and analyzed by

Surface plasmon resonance microscopy: achieving a quantitative optical response

September 8, 2016
Author(s)
Alexander W. Peterson, Michael W. Halter, Anne L. Plant, John T. Elliott
Surface plasmon resonance imaging (SPRI) allows real-time label-free imaging based on index of refraction, and changes in index of refraction at an interface. SPRI can be carried out on a microscope by launching light into a sample, and collecting

Inhibition of DNA glycosylases in development of cancer therapeutics

September 4, 2016
Author(s)
M Miral Dizdar, Pawel Jaruga, Erdem Coskun, Marcus J. Calkins, Nathan Donley, Dorjbal Dorjsuren, Anton Simeonov, Amanda K. McCullough, R S. Lloyd, Ajit Jadhav
In cancer therapy, the efficacy of therapeutic agents may be influenced by increased DNA repair capacity. This may be due to overexpression of DNA repair proteins that repair therapy-induced DNA lesions in tumors before they become toxic. Inhibition of DNA

Certified DNA Reference Materials to Compare HER2 Gene Amplification Measurements Using Next- Generation Sequencing Methods

September 1, 2016
Author(s)
Chih-Jian Lih, Robin D. Harrington, Kneshay Harper, David J. Sims, Paul McGregor, Corinne Camalier, Han Si, Biswajit Das, P. M. Williams, Hua-Jun He, Jamie L. Almeida, Steven Lund, Steven J. Choquette, Kenneth D. Cole
The National Institute of Standards and Technology (NIST) Standard Reference Materials 2373 is a set of genomic DNA samples prepared from five breast cancer cell lines with certified values for the ratio of the HER2 gene copy number to the copy numbers of

The Comet Assay: Methods for Quantitative Image Analysis and Reproducibility

September 1, 2016
Author(s)
Donald H. Atha, Signe Braafladt, Vytautas Reipa
The comet assay,or single cell gel electrophoresis,is a sensitive and efficient technique for analyzing DNA damage in the form of single and double strand breaks in single cells. DNA alteration,from strand breaks,can lead to cancer and other health risks

NIST Micronutrients Measurement Quality Assurance Program Winter, Spring, and Fall 1988 Comparability Studies: Results for Round Robins XII, XIII, and XIV Fat-Soluble Vitamins and Carotenoids in Human Serum

August 29, 2016
Author(s)
Jeanice M. Brown Thomas, David L. Duewer, Margaret C. Kline, Willie E. May
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat-soluble vitamins and carotenoids in human serum and plasma. This report describes the design

NIST Micronutrients Measurement Quality Assurance Program Winter, Spring, and Fall 1989 Comparability Studies: Results for Round Robins XV, XVI, and XVII Fat-Soluble Vitamins and Carotenoids in Human Serum

August 29, 2016
Author(s)
Jeanice M. Brown Thomas, David L. Duewer, Margaret C. Kline, Willie E. May, Neal E. Craft
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat -soluble vitamins and carotenoids in human serum and plasma. This report describes the design

Roles of Nanofiber Scaffold Structure and Chemistry in Directing Human Bone Marrow Stromal Cell Response

August 22, 2016
Author(s)
Sumona Sarkar, Bryan A. Baker, Desu Chen, Patrick S. Pine, Jennifer H. McDaniel, Marc L. Salit, Wolfgang Losert, Carl G. Simon Jr., Joy P. Dunkers
Nanofiber technology has emerged as a promising tool to recapitulate the native extracellular matrix structure; however the properties of nanofibers governing cell-material interactions are still largely undetermined. In this study we have systematically

NIST Micronutrients Measurement Quality Assurance Program Summer 2015 Comparability Studies: Results for Round Robin LXXVIII Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 43 Ascorbic Acid in Human Serum

July 28, 2016
Author(s)
Jeanice M. Brown Thomas, David L. Duewer
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

NIST Micronutrients Measurement Quality Assurance Program Winter 2015 Comparability Studies: Results for Round Robin LXXVII Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 42 Ascorbic Acid in Human Serum

July 28, 2016
Author(s)
Jeanice M. Brown Thomas, David L. Duewer
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

A New Molecular Model for Lithium's Biological Interactions

July 25, 2016
Author(s)
John Marino, Katharine T. Briggs, Gary G. Giulian, Joe Kao, Gong Li
Lithium carbonate, a drug for the treatment of Bipolar Disorder, provides mood stability to mitigate recurrent episodes of mania and/or depression. Despite its long term and widespread use, the mechanism by which lithium acts to elicit these psychological

Anti-corrosive influence of Acetobacter biofilms on carbon steel

July 19, 2016
Author(s)
Danielle C. France
Microbiologically influenced corrosion (MIC) of carbon steel infrastructure is an emerging cost issue for the ethanol fuel industry. One bacterial species found in ethanol fuel environments, Acetobacter aceti, can convert ethanol to the corrosive species

Machine Learning Strategy to Determine Cell Shape Phenotypes Associated with Micro-Environmental Cues

June 25, 2016
Author(s)
Desu Chen, Sumona Sarkar, Julian Candia, Carl G. Simon Jr., Wolfgang Losert, Joy P. Dunkers, Stephen J. Florczyk, Subhadip Bodhak, Meghan Driscoll
Cell morphology has been identified as a potential indicator of stem cell response and function in biomaterial environments. However, determination of cell shape phenotype in biomaterials is complicated by heterogeneous cell populations, heterogeneous
Displaying 351 - 375 of 1174