U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications

NIST Authors in Bold

Displaying 351 - 375 of 837

High Resolution Surface Plasmon Resonance Imaging for Single Cells

November 30, 2014
Author(s)
Alexander Peterson, Michael Halter, Alessandro Tona, Anne Plant
Background Surface plasmon resonance imaging (SPRI) is a label-free technique that can image refractive index changes at an interface. We have previously shown that SPRI can be used to study the dynamics of cell-substratum interactions. However

Oxidatively Induced DNA Damage and Its Repair in Cancer

November 25, 2014
Author(s)
M Miral Dizdar
Oxidatively induced DNA damage is caused in living organisms by endogenous and exogenous reactive species. DNA lesions resulting from this type of damage are mutagenic and cytotoxic and, if not repaired, can cause genetic instability that may lead to

Genomic Reference Materials for Clinical Application

November 21, 2014
Author(s)
Justin M. Zook, Marc L. Salit
Reference Materials are well-characterized, homogeneous, and stable samples that can be used to understand measurement performance. The Genome in a Bottle Consortium is developing whole human genome DNA Reference Materials from large batches of DNA

Accurate determination of the size distribution for polydisperse, cationic metallic nanomaterials by asymmetric-flow field flow fractionation

November 13, 2014
Author(s)
Julien C. Gigault, Thao M. Nguyen, John M. Pettibone, Vincent A. Hackley
In this work we have developed and validated a methodology for determining the size distribution in polydisperse cationic nanoparticle (NP) samples using asymmetric-flow field flow fractionation (A4F), where known compositional influence of previously used

Archaeal DNA replication

November 3, 2014
Author(s)
Zvi Kelman, Lori M. Kelman
DNA replication is an essential process for all life forms. While the process is fundamentally conserved in the three domains of life, bioinformatics, biochemical, structural and genetic studies have demonstrated that the process and the proteins involved

Assessing technical performance in differential gene expression experiments with external spike- in RNA control ratio mixtures

September 25, 2014
Author(s)
Sarah A. Munro, Steven P. Lund, Patrick S. Pine, Hans Binder, Djork-Arne Clevert, Ana Conesa, Joaquin Dopazo, Mario Fasold, Sepp Hochreiter, Huixao Hong, Nederah Jafari, David P. Kreil, Pawel P. Labaj, Yang Liao, Simon Lin, Christopher E. Mason, Javier Santoyo, Steven J. Schrodi, Leming Shi, Wei Shi, Gordon K. Smyth, Nancy Stralis-Pavese, Zhenqiang Su, Charles Wang, Jian Wang, Joshua Xu, Zhan Ye, Yong Yang, Ying Yu, Paul Zumbo, Marc L. Salit
There is a critical need for standard approaches to assess, report and compare the technical performance of genome-scale differential gene expression experiments. Here we assess technical performance with a proposed standard ‘dashboard’ of metrics derived

An Average Enumeration Method of Hyperspectral Imaging Data for Quantitative Evaluation of Medical Device Surface Contamination

September 15, 2014
Author(s)
Hanh Le, Moon Kim, Jeeseong C. Hwang, Yi Yang, Pawneena U-Thainual, Jin Kang, Do-Hyun Kim
We propose a quantification method called Mapped Average Principal component analysis Score (MAPS) to enumerate the contamination coverage on common medical device surfaces. The method was adapted from conventional Principal Component Analysis (PCA) on non

Rbm24a and Rbm24b are required for normal somitogenesis

August 29, 2014
Author(s)
Samantha D. Maragh
We recently demonstrated that the gene encoding the RNA binding motif protein 24 (Rbm24)¬¬ is expressed during mouse cardiogenesis, and determined the developmental requirement for its zebrafish homologs Rbm24a and Rbm24b during cardiac development. We

Assuring Confidence in Measurements in Biomedical Research

August 28, 2014
Author(s)
Anne L. Plant, Laurie E. Locascio, Willie E. May, Patrick D. Gallagher
‘Irreproducibility’ is a symptom of a broad challenge in measurement that needs to be addressed. Establishing a robust measurement process that will lead to greater confidence in biomedical research results will require a concerted effort from experts.

Multiscale Correlative Measurements of Nanoparticles in Cells

August 26, 2014
Author(s)
Aric W. Sanders, Kavita M. Jeerage, Cindi Schwartz, Alexandra E. Curtin, Ann C. Chiaramonti Debay
Nanoparticles are emerging as invaluable tools in disease diagnosis, disease treatment and imaging contrast enhancement agents. The interactions of nanoparticles with host organisms are complex and affect biological systems over length scales that vary

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope Using Fluorescent Glass

August 11, 2014
Author(s)
Michael W. Halter, Elianna Bier, Paul C. DeRose, Gregory A. Cooksey, Steven J. Choquette, Anne L. Plant, John T. Elliott
Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other screening applications, few published methods exist for

Flow Cytometer Performance Characterization, Standardization and Calibration against CD4 on T Lymphocytes Enables Quantification of Biomarker Expressions for Immunological Applications

August 7, 2014
Author(s)
Heba Degheidy, Steven R. Bauer, Gerald Marti, Lili Wang
There is an urgent need for developing a procedure for biomarker standardization and quantification in clinical laboratories. Measuring the expression levels of cell antigens is critical for the diagnosis of many diseases, e.g. leukemia, lymphoma and

Membrane protein resistance of oligo(ethylene oxide) self-assembled monolayers

July 31, 2014
Author(s)
David J. Vanderah, Marlon L. Walker, David T. Gallagher, Ryan Vierling, Fay Crawshaw
Spectroscopic ellipsometry was used to evaluate the resistance to protein adsorption (RPA) of self- assembled monolayers (SAMs) of HS(CH2)3O(CH2CH2O)6M and [HS(CH2)3CH]2O-(CH2CH2O)6M, where M = CH3 or H, on Au. The SAMs were exposed to fibrinogen, a

High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues

July 20, 2014
Author(s)
Charles H. Camp, Young J. Lee, John M. Heddleston, Christopher M. Hartshorn, Angela R. Hight Walker, Jeremy N. Rich, Justin D. Lathia, Marcus T. Cicerone
We have developed a coherent Raman imaging platform using broadband coherent anti-Stokes Raman scattering (BCARS) that provides an unprecedented combination of speed, sensitivity, and spectral breadth. The system utilizes a unique configuration of laser

Spectral Fluorescence Measurements

July 7, 2014
Author(s)
Paul C. DeRose, Joanne C. Zwinkels, James Leland
This chapter covers the topic of spectral fluorescence measurements. These fluorescence measurements offer significant advantages in terms of sensitivity and selectivity, finding wide use in a range of applications in analytical and color technologies. The

Viscosity Measurements of DNA Solutions with and without Condensing Agents

July 7, 2014
Author(s)
Arno D. Laesecke, Jessica L. Burger
Aqueous DNA solutions at three concentrations were studied as model sputum by viscosity measurements at ambient pressure. Initial measurements at 25 °C, 37.78 °C, and 50 °C indicated a viscosity increase toward 50 °C. This phenomenon was studied by

State of the Art Raman Techniques for Biological Applications

July 1, 2014
Author(s)
Angela R. Hight Walker, Alasdair Rae, Rainer Stosch, Petr Klapetek, Debdulal Roy
Raman spectroscopy is a powerful tool for the elucidation of qualitative and quantitative information from biological systems and has huge potential in areas such as biotechnologies, drug discovery, agro-chemical research and clinical diagnostics. This

Standards in Biothermodynamics

May 15, 2014
Author(s)
Robert N. Goldberg
The field of biothermodynamics encompasses physical property measurements on biochemical and biological systems. This chapter reviews the status of standards documents that are pertinent to biothermodynamics as well as recommendations that have been made

Machine Learning Analysis for Identification of Cell Shape Metrics Associated with Stem Cell Differentiation in Nanofiber Scaffolds

April 19, 2014
Author(s)
Desu Chen, Sumona Sarkar, Stephen J. Florczyk, Subhadip Bodhak, Carl G. Simon Jr., Joy P. Dunkers, Julian Candia, Wolfgang Losert
Cell shape has been demonstrated to be closely related to cell function and may be an important predictor of cell fate. Many metrics are available to describe cell shape, however the relationship of these metrics to cell fate are not well understood

Measuring Organelle Shape in 3D in Stem Cells Cultured on Nanofiber Scaffolds

April 19, 2014
Author(s)
Carl G. Simon Jr., Peter Bajcsy, Wojtek J. Tutak, Jyotsnendu J. Giri
Previous work has demonstrated that culture of osteoprogenitor cells on nanofiber scaffolds can potentiate osteogenic differentiation [1-4]. Culture of cells in nanofiber scaffolds causes changes to cell morphology, suggesting that morphological changes
Displaying 351 - 375 of 837