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Displaying 376 - 400 of 837

Structural Basis for Inactivation of Giardia lamblia Carbamate Kinase by Disulfiram

April 11, 2014
Author(s)
Andrey Galkin, Liudmila Kulakova, Kap Lim, Catherine Chen, Wei Zhang, Illarion Turko, Osnat Herzberg
Carbamate kinase from Giardia lamblia is an essential enzyme for the survival of the organism. The enzyme catalyzes the final step in the arginine dihydrolase pathway converting ADP and carbamoyl phosphate to ATP and carbamate. We previously reported that

AN EXAMINATION OF METAL DEPOSITION DERIVED FROM AEROSPACE-RELATED ACTIVITY IN SEVERAL ENVIRONMENTAL MATRICES COLLECTED FROM WATERS SURROUNDING THE KENNEDY SPACE CENTER (KSC), FLORIDA

April 8, 2014
Author(s)
John Bowden, Theresa M. Cantu, Douglas M. Scheidt, Russell H. Lowers, Brian A. Nocito, Vaneica Y. Young, Louis J. Guillette
Metal deposition originating from anthropogenic activity was examined in a two-tier study using environmental samples collected from several locations at the Kennedy Space Center (KSC) and surrounding Merritt Island National Wildlife Refuge (MINWR) with

Measuring Stem Cell Dimensionality in Tissue Scaffolds

April 1, 2014
Author(s)
Carl G. Simon Jr., Sapun Parekh, Christopher K. Tison, Girish Kumar, Tanya M. Farooque, Charles H. Camp
Many 3D scaffold systems have evolved for tissue engineering, drug screening and in vitro tissue models. However, it is not clear which scaffolds provide a 3D cell niche and there is no clear way to measure cell niche dimensionality. Advances in 3D niche

Application of Falling-Needle Rheometry to Highly Concentrated DNA Solutions.

March 18, 2014
Author(s)
Jessica L. Burger, Hideki Yamamoto, Takamasa Suzuki, Arno D. Laesecke
High concentration DNA solutions are common both in vitro and in vivo, and understanding the rheological properties is a critical area of bioscience. Our previous measurements on high concentration DNA solutions (2-6 mg/ml) interestingly provided evidence

Certification of Total Arsenic in Blood and Urine Standard Reference Materials by Radiochemical Neutron Activation Analysis and Inductively Coupled Plasma Mass Spectrometry

March 1, 2014
Author(s)
Rick L. Paul, William C. Davis, Karen E. Murphy, Colleen E. Bryan Sallee, Lee L. Yu, William F. Guthrie, Dennis D. Leber, Thomas W. Vetter
A newly developed procedure for determination of arsenic by radiochemical neutron activation analysis was used to measure arsenic in SRM 955c Toxic Elements in Caprine Blood and SRM 2668 Toxic Elements in Frozen Human Urine for the purpose of providing

CPTAC Studies 1 and 5: dissecting variability in multi-site LC-MS/MS proteomics through quality metrics and robust hierarchical multivariate statistics

February 4, 2014
Author(s)
Stephen E. Stein, David M. Bunk, Xia Wang, Matthew Chambers, Lorenzo J. Vega-Montoto, David L. Tabb
Shotgun proteomics experiments integrate a complex sequence of processes, any of which can introduce variability. Quality metrics computed from LC-MS/MS data have relied upon identifying MS/MS scans, but a new mode for the QuaMeter software produces

Influence of tissue, age, and environmental quality on global levels of genomic methylation in the American alligator (Alligator mississippiensis)

January 7, 2014
Author(s)
Benjamin B. Parrott, John Bowden, Satomi Kohno, Jessica A. Cloy-McCoy, Jacqueline T. Bangma, Thomas R. Rainwater, Phillip M. Wilkinson, John Kucklick, Louis J. Guillette
Epigenetic modifications are key mediators of the interactions between the environment and an organism's genome. DNA methylation represents the best-studied epigenetic modification to date and is known to play key roles in regulating transcriptional

Probing the intracellular glutathione redox potential using in-cell NMR spectroscopy

January 2, 2014
Author(s)
Steve Y. Rhieu, Aaron A. Urbas, Daniel W. Bearden, John P. Marino, Vytautas Reipa, Katrice A. Lippa
Non-invasive and real-time analysis of cellular redox processes has been greatly hampered by lack of suitable measurement techniques. Here we describe an in-cell nuclear magnetic resonance (NMR)-based method for measuring the intracellular glutathione

Dielectrophoretic Trapping of P19 Cells on Indium Tin Oxide based Microelectrode Arrays

November 8, 2013
Author(s)
Aveek Gangopadhyay, Saugandhika Minnikanti, Darwin Reyes-Hernandez, Mulpuri V. Rao, Nathalia Peixoto
A microfabricated device comprised of a microelectrode array (MEA) and a microfluidic channel is presented here for the purpose of trapping cells using positive dielectrophoresis (DEP). Transparent indium tin oxide (ITO) electrodes are patterned in an

Airbrushed Nanofiber Scaffolds Support Bone Marrow Stromal Cell Differentiation

November 1, 2013
Author(s)
Carl G. Simon Jr., Wojtek J. Tutak, Sumona Sarkar, Tanya M. Farooque, Jyotsnendu J. Giri, Dongbo Wang, Sheng Lin-Gibson, Joachim Kohn, Durgadas Bolikal
Nanofiber scaffolds are effective for tissue engineering since they emulate the fibrous nanostructure of native extracellular matrix (ECM). Although electrospinning has been the most common approach for fabricating nanofiber scaffolds, airbrushing

Determining carbapenemase activity with 18O labeling and targeted mass spectrometry

October 16, 2013
Author(s)
Meiyao Wang, Yang Shen, Illarion Turko, Daniel Nelson, Shuwei Li
Carbapenems are broad spectrum antibiotics considered as last resort drugs to treat bacterial infections. Carbapenem-hydrolyzing β-lactamases (also called carbapenemases), however, can confer bacterial resistance and represent a serious health threat. Here

Comprehensive analysis of protein digestion using six trypsins reveals the origin of trypsin as a significant source of variability in proteomics

October 11, 2013
Author(s)
Stephen E. Stein, Paul A. Rudnick, Scott J. Walmsley, Qian Dong, Alexey I. Nesvizhskii, Yuxue Liang
Trypsin is an endoprotease commonly used for sample preparation in proteomics experiments. Importantly, protein digestion is dependent on multiple factors, including the trypsin origin and digestion conditions. In-depth characterization of trypsin activity

Functionalization-Dependent Induction of Cellular Survival Pathways by CdSe Quantum Dots in Primary Normal Human Bronchial Epithelial Cells

September 5, 2013
Author(s)
Amber Nagy, Jennifer A. Hollingsworth, Bin Hu, Andrea Steinbruck, Peter C. Stark, Cristina Rios Valdez, Momchilo Vuyisich, Michael H. Stewart, Donald H. Atha, Bryant C. Nelson, Rashi Iyer
Quantum dots (QDs) are semiconductor nanocrystals exhibiting unique optical properties which can be exploited for many practical applications ranging from photovoltaics to biomedical imaging and drug delivery. They are increasingly manufactured in large

NIST Micronutrients Measurement Quality Assurance Program Summer 2007 Comparability Studies: Results for Round Robin LXII Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 27 Ascorbic Acid in Human Serum

August 5, 2013
Author(s)
David L. Duewer, Jeanice M. Brown Thomas
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

NIST Micronutrients Measurement Quality Assurance Program Summer 2008 Comparability Studies: Results for Round Robin LXIV Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 29 Ascorbic Acid in Human Serum

August 5, 2013
Author(s)
David L. Duewer, Jeanice M. Brown Thomas
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

NIST Micronutrients Measurement Quality Assurance Program Summer 2009 Comparability Studies: Results for Round Robin LXVI Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 31 Ascorbic Acid in Human Serum

August 5, 2013
Author(s)
David L. Duewer, Jeanice M. Brown Thomas
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes
Displaying 376 - 400 of 837