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Displaying 551 - 575 of 1174

Anchor Molecules Affect Structure and Properties of Tethered Bilayer Lipid Membranes. Unsaturation in the Alkyl Chains.

June 7, 2013
Author(s)
David J. Vanderah, Rima Budvytyte, Gintaras Valincius, Gediminas Niaura, Vladislava Voiciuk, Mindaugas Mickevicius, Haw-Zan Goh, Hilary Stauffer, Frank Heinrich, Siddharth Shenoy
The self-assembled monolayers (SAMs) of the new lipidic anchor molecule, Z 20-(Z-octadec-9-enyloxy)-3,6,9,12,15,18,22-heptaoxatetracont-31-ene-1-thiol (HC18), and mixed HC18/-mercaptoethanol (ME) SAMs were studied by spectroscopic ellipsometry, contact

Controlled Chemistry for Studying Combined Effects of Extracellular Matrix Proteins

April 22, 2013
Author(s)
Antony K. Chen, Frank W. DelRio, Alexander W. Peterson, Koo-Hyun Chung, Kiran Bhadriraju, Anne L. Plant
The extracellular matrix (ECM) consists of a complex mixture of biochemical and physical stimuli that together regulate cell behavior. Previously we showed that films composed of Type I collagen fibrils induce reproducible and physiologically relevant cell

Effect of Surface Chemistry on Stem Cell Response in 2-D vs. 3-D Cell Culture Niches

April 19, 2013
Author(s)
Sumona Sarkar, Carl G. Simon Jr., Roberta I. Lock, Joy P. Dunkers
To develop tissue engineered devices for regenerative medicine strategies, key scaffold properties for directing stem cell response must be identified. As focus shifts from 2-D to 3-D culture systems in an attempt to better recapitulate the natural

Active Transcriptomic and Proteomic Reprogramming in the C. elegans Nucleotide Excision Repair Mutant xpa-1

April 10, 2013
Author(s)
Katarzyna Arczewska, Gisele Tomazella, Jessica Lindvall, Henok Kassahun, Silvia Maglioni, Alessandro Torgovnick, Johan Henriksson, Olli Matilainen, Bryce J. Marquis, Bryant C. Nelson, Eshrat Babaie, Carina Holmberg, Thomas Burglin, Natascia Ventura, Bernd Thiede, Hilde Nilsen
Transcription-blocking oxidative DNA damage is believed to contribute to aging and to underlie activation of oxidative stress-responses and down-regulation of insulin-like signaling (ILS) in Nucleotide Excision Repair (NER) deficient mice. Here, we present

Guidelines for Minimum Information for Publication of Quantitative Digital PCR Experiments

April 9, 2013
Author(s)
Ross J. Haynes, Jim Huggett, Carole Foy, Vladimir Benes, Kerry Emslie, Jeremy Garson, Jan Hellemans, Mikael Kubista, Tania Nolan, Michael Pfaffl, Gregory Shipley, Jo Vandesompele, Carl Wittwer, Stephen Bustin
There is growing interest in the digital polymerase chain reaction (dPCR) as technological progress makes it a practical and increasingly affordable technology. dPCR allows the precise quantification of nucleic acid, facilitating the measurement of small

Salivary Antigen-5/CAP Family Members Are Cu 2+ -dependent Antioxidant Enzymes That Scavenge O 2 -. and Inhibit Collagen-induced Platelet Aggregation and Neutrophil Oxidative Burst

April 4, 2013
Author(s)
Teresa Assumpcao, Dongying Ma, Alexandra Schwarz, Karine Reiter, Jaime Santana, John Andersen, Jose Ribeiro, Glen Nardone, Lee L. Yu, Ivo Francischetti
The function of the antigen-5/CAP family of proteins found in the salivary gland of bloodsucking animals has remained elusive for decades. Antigen-5 members from the hematophagous insects Dipetalogaster maxima (DMAV) and Triatoma infestans (TIAV) were

Spectral Light source Distribution variations to enhance discrimination of the common bile duct from surroundings in reflectance hyperspectral imaging

March 8, 2013
Author(s)
Maritoni A. Litorja, Mira Fein, Eleanor Wehner, Karel Zuzak, Edward Livingston
The classification of anatomical features using hyperspectral imaging has been a common goal in biomedical hyperspectral imaging. Identification and location of the common bile duct is critical in cholecystectomies, one of the most common surgical

Gradient Nanofiber Scaffold Libraries for Screening Cell Response to Poly(e-caprolactone)-Calcium Phosphate Composites

March 1, 2013
Author(s)
Carl G. Simon Jr., Limin Sun, Laurence C. Chow, William Miles, Christopher K. Tison, Kaushik Chatterjee, Marian F. Young, Vinoy Thomas, Murugan Ramalingam
A 2-spinnerette approach has been developed for fabricating nanofiber scaffold gradients for use as 1) scaffold libraries for screening the effect of nanofiber properties on cell response and 2) templates for generating graded tissue interfaces. Although

Standard Reference Material 2366 for Measurement of Human Cytomegalovirus DNA

March 1, 2013
Author(s)
Ross J. Haynes, Margaret C. Kline, Blaza Toman, Calum Scott, Paul Wallace, John M. Butler, Marcia J. Holden
Cytomegalovirus (CMV) is one of a number of human viruses that are ubiquitous in human populations, generally cause little illness upon infecting healthy individuals, but can cause life threatening disease in immune-compromised individuals. An important

Modification of tethered bilayers by phospholipid exchange with vesicles

February 27, 2013
Author(s)
David J. Vanderah, Rima Budvytyte, Mindaugas Mickevicius, Gintaras Valincius, Frank Heinrich
Phosphatidylcholine and cholesterol exchange between vesicles and planar tethered bilayer lipid membranes (tBLMs) was demonstrated from electrochemical impedance spectroscopy (EIS), fluorescence microscopy (FM), and neutron reflectometry (NR) data

Mouse Cell Line Authentication

February 13, 2013
Author(s)
Jamie L. Almeida, Carolyn R. Steffen, Kenneth D. Cole
The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells; however, there are few assays available for nonhuman cell line identification. We have developed a multiplex polymerase

Thermococcus kodakarensis DNA replication

February 2, 2013
Author(s)
Zvi Kelman, Lori Kelman, Zhuo Li
DNA replication plays an essential role in all life forms. Research on archaeal DNA replication began about twenty years ago. Progress was hindered, however, by the lack of genetic tools to supplement the biochemical and structural studies. This has

Further Studies with Isolated Absolute Infrared Spectra of Bacteriorhodopsin Photocycle Intermediates: Conformational Changes and Possible Role of a New Proton-Binding Center

January 1, 2013
Author(s)
Richard W. Hendler, Curtis W. Meuse, Paul Smith, John W. Kakareka
We recently published procedures describing the isolation of absolute infrared spectra for the intermediates of the bacteriorhodopsin (BR) photocycle and from these, obtaining transitional difference spectra between consecutive intermediates. In that work

Identification and Quantification of Human DNA Repair Protein NEIL1 by Liquid Chromatography/Isotope-Dilution Tandem Mass Spectrometry

December 26, 2012
Author(s)
M Miral Dizdar, Pawel Jaruga, Guldal Kirkali, Bryant C. Nelson, Gamze Tuna, Prasad T. Reddy
Accumulated evidence points to DNA repair capacity as an important factor in cancer and other diseases. DNA repair proteins are promising drug targets and are emerging as prognostic and therapeutic biomarkers. Thus, the knowledge of the overexpression or
Displaying 551 - 575 of 1174