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Displaying 576 - 600 of 1174

Structure and Activity of PA5508, a Novel Hexameric Glutamine Synthase Homolog

December 12, 2012
Author(s)
Jane E. Ladner, James F. Parsons
The structure of PA5508 from Pseudomonas aeruginosa, a glutamine synthase (GS) homolog, has been solved at 2.5 Å. Surprisingly, PA5508 forms single hexameric rings rather than the stacked double rings that are characteristic of GS. The C-terminal helical

The Effects of Diffusion on an Exonuclease/Nanopore-Based DNA Sequencing Engine

December 7, 2012
Author(s)
Joseph E. Reiner, Joseph W. Robertson, Arvind Balijepalli, Daniel L. Burden, Bryon S. Drown, John J. Kasianowicz
The ability to electronically detect and characterize individual polynucleotides as they are driven through a single protein ion channel may eventually prove useful for rapidly sequencing DNA (base-by-base) in a ticker tape-like fashion. More recently, a

Relative Rigidity of Cell-Substrate Affects Hepatic and Hepatocellular Carcinoma Cell Migration

December 3, 2012
Author(s)
Martin Y. Chiang, Hongbing Wang, Li Zhong, Qiaoyan Tan, Gurinder K. Singh, Song Li, Li Yang, Yanzi Yangben
Polyacrylamide gels with different stiffness and glass were employed as substrates to investigate how substrate stiffness affects the cellular stiffness of adherent hepatocellular carcinoma (HCCLM3) and hepatic (L02) cells. The interaction of how cell

Immobilization of Proteins on Carboxylic Acid-Functionalized Nanopatterns

November 28, 2012
Author(s)
John Peter Ngunjiri, Daniel J. Stark, Tian Tian, Kimberly Briggman, Jayne C. Garno
The immobilization of proteins on nanopatterned surfaces was investigated using in situ atomic force microscopy (AFM) and ex situ infrared reflectance-absorption spectroscopy (IRAS). The AFM-based lithography technique of nanografting provided control of

Remote sensing of chiral signatures on Mars

November 1, 2012
Author(s)
William Sparks, James H. Hough, Thomas Germer, Frank Robb, Ludmilla Kolokolova
We describe circular polarization as a remote sensing diagnostic of chiral signatures which may be applied to Mars. The remarkable phenomenon of homochirality provides a unique biosignature which can be amenable to remote sensing through circular

Toward a Recommendation System for Image Similarity Metrics

October 7, 2012
Author(s)
Peter Bajcsy, Joe Chalfoun, Mary C. Brady
This paper addresses the problem of mapping application specific requirements on image similarity metrics to the plethora of existing image similarity computations. The work is motivated by the fact that there is no recommendation method for choosing a

PEG-Labeled Nucleotides and Nanopore Detection for Single Molecule DNA Sequencing by Synthesis

September 21, 2012
Author(s)
Shiv Kumar, Tao Chuanjuan, Chien Minchen, Hellner Brittney, Joseph W. Robertson, Arvind Balijepalli, Li Zengmin, James J. Russo, Joseph E. Reiner, John J. Kasianowicz, Jingyue Ju
There is a significant need to accurately sequence single DNA and RNA molecules for personalized medicine. We describe a novel nanopore-based sequencing by synthesis (SBS) strategy that will accurately differentiate at single molecule level four different

Evaluation of viability and proliferation profiles on macrophages treated with silica nanoparticles in vitro via plate-based, flow cytometry, and Coulter counter assays

September 3, 2012
Author(s)
Simona Bancos, De-Hao D. Tsai, Vincent A. Hackley, J L. Weaver, Katherine M. Tyner
Nanoparticles (NPs) are known to interfere with many high throughput cell viability and cell proliferation assays, which complicates the assessment of their potential toxic effects. The aim of this study was to compare viability and proliferation results

Disentangling the effects of polymer coatings on silver nanoparticle agglomeration, dissolution, and toxicity to determine mechanisms of nanotoxicity

September 1, 2012
Author(s)
Justin M. Zook, Melissa D. Halter, Danielle Cleveland, Stephen E. Long
Silver nanoparticles (AgNPs) are frequently coated by a variety of polymers, which may affect various intertwined mechanisms of toxicity, including agglomeration and dissolution rate. Here, we measure how citrate, dextran, poly(ethylene glycol) (PEG)

Review: Biological Imaging Software Tools

August 29, 2012
Author(s)
Anne L. Plant, Kevin Eliceiri, Anne Carpenter
Few technologies are more widespread in modern biological laboratories than imaging by microscopy. Advancements in optical technologies and instrumentation over the past twenty years have led to massive improvements in the resolution, specificity

Quantitative Methods to Characterize Cell Lines: Comparison of Cells from Marine and Terrestrial Mammals.

July 28, 2012
Author(s)
Tighe Spurlin, John T. Elliott, Michael W. Halter, Kiran Bhadriraju, Alessandro Tona, Anne L. Plant, Annalaura Mancia, Bobby L. Middlebrooks, Gregory W. Warr
Descriptive terms are often used to characterize cells in culture, but the use of nonquantitative and poorly defined terms can lead to ambiguities when comparing data from different laboratories. Although recently there has been a good deal of interest in

A New Measure in Cell Image Segmentation Data Analysis

July 24, 2012
Author(s)
Jin Chu Wu, Michael W. Halter, Raghu N. Kacker, John T. Elliott
Cell image segmentation (CIS) is critical for quantitative imaging in cytometric analyses. The data derived after segmentation can be used to infer cellular function. To evaluate CIS algorithms, first for dealing with comparisons of single cells treated as
Displaying 576 - 600 of 1174