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Displaying 576 - 600 of 838

Differences in PDMS Modification Affect Laminin Deposition and Smooth Muscle Cell Response

August 16, 2009
Author(s)
Joy P. Dunkers, Hae-Jeong Lee, Marvi Matos, Lisa Pakstis, Juan M. Taboas, Steven D. Hudson, Marcus T. Cicerone
When culturing cells on flexible surfaces, it is important to consider extracellular matrix treatments that will remain on the surface under mechanical strain. Here we investigate differences in laminin surfaces that were deposited on oxidized PDMS (plasma

Protein crystal engineering of YpAC-IV using a strategy of excess charge reduction

August 5, 2009
Author(s)
David T. Gallagher, N N. Smith, Sung Kim, Howard Robinson, Prasad T. Reddy
The class IV adenylyl cyclase from Yersinia pestis has been engineered to enable crystallization at neutral pH for mechanism studies. The wild-type enzyme crystallized only at pH below 5. Based on the unliganded wild-type structure 2FJT at 1.9 Angstrom

Synthetic Lung Tumor Data Sets for Comparison of Volumetric Algorithms

July 13, 2009
Author(s)
Adele P. Peskin, Alden A. Dima, Javier Bernal, David E. Gilsinn, Karen Kafadar
The change in pulmonary nodules over time is an important indicator of malignant tumors. It is therefore important to be able to measure change in the size of tumors from computed tomography (CT) data taken at different times and on potentially different

Selective Binding of RNAse B Glycoforms by Polydopamine-immobilized Concanavalin A

June 10, 2009
Author(s)
Todd A. Morris, Alexander W. Peterson, Michael J. Tarlov
Glycoanalysis is important in the manufacture and quality control of protein therapeutics. An emerging method for glycoanalysis is the use of lectin arrays. Critical to the performance of these arrays is the immobilization of the lectin molecules

3D Imaging of Diatoms with Ion-abrasion Scanning Electron Microscopy

June 9, 2009
Author(s)
Keana C. Scott, Mark Hildebrand, Sang Kim, Dan Shi, Sriram Subramaniam
Ion-abrasion scanning electron microscopy (IASEM) takes advantage of focused ion beams to abrade thin sections from the surface of bulk specimens, coupled with SEM to image the surface of each section, enabling 3D reconstructions of subcellular

Lambda exonuclease digestion of CGG trinucleotide repeats

June 7, 2009
Author(s)
Richard Conroy, Alan Koretsky, John M. Moreland
Fragile X syndrome and other trinucleotide diseases are characterized by an elongation of a repeating DNA triplet. The ensemble-averaged lambda exonuclease digestion rate of diVerent substrates, including one with an elongated FMR1 gene containing 120 CGG

T7-Based Linear Amplification of Low Concentration mRNA Samples using Beads and Microfluidics for Global Gene Expression Measurements

April 7, 2009
Author(s)
Jason G. Kralj, Audrey Player, Hope Sedrick, Matt S. Munson, David Petersen, Samuel P. Forry, Paul Meltzer, Ernest Kawasaki, Laurie E. Locascio
We show single-cell level linear amplification of gene expression libraries immobilized on a microfluidic packed bed. This is a critical step in the Eberwine process, which is used to amplify messenger RNA (mRNA) for gene expression profiling. Microarray

Polymerization Stress Development in Dental Composites: Effect of Cavity Design Factor

March 13, 2009
Author(s)
Joseph M. Antonucci, Anthony A. Giuseppetti, Justin N. O'Donnell, Gary E. Schumacher, Drago Skrtic
Objective: To assess the effect of the cavity design factor (C-factor) on polymerization stress development (PSD) in resin composites. Methods: An experimental resin (BT resin) was prepared, which contained 2,2-bis[p-(2 hydroxy-3 -methacryloxypropoxy

Cell volume distributions reveal cell growth rates and division times

March 7, 2009
Author(s)
Michael W. Halter, John T. Elliott, Joseph B. Hubbard, Alessandro Tona, Anne L. Plant
A population of cells in culture displays a range of phenotypic responses, even when those cells are derived from a single cell and are exposed to a homogeneous environment. Phenotypic variability can have a number of sources, including the variable rates

Surface plasmon resonance imaging of cells and surface-associated fibronectin

February 26, 2009
Author(s)
Alexander W. Peterson, Michael W. Halter, Alessandro Tona, Kiran Bhadriraju, Anne L. Plant
Background A critical challenge in cell biology is quantifying the interactions of cells with their extracellular matrix (ECM) environment and the active remodeling by cells of their ECM. Fluorescence microscopy is a commonly employed technique for

Equilibrium Electrostatics of Responsive Polyelectrolyte Monolayers

January 14, 2009
Author(s)
Rebecca A. Zangmeister, Rastislav Levicky, Kang Wang
The physical behavior of polyelectrolytes at solid-liquid interfaces presents challenges both in measurement and in interpretation. An informative, yet often overlooked, property that characterizes the equilibrium organization of these systems is their

Robotic Patient Lift and Transfer

December 31, 2008
Author(s)
Roger V. Bostelman, James S. Albus
This book chapter provides background on wheelchairs, patient lift devices and the combination of mobile patient lift and transfer devices and research. The NIST Home Lift, Position and Rehabilitation (HLPR) Chair is detailed explaining the system, its use

The Role for Engineers in Health Care Information Systems

December 30, 2008
Author(s)
Ram D. Sriram, Steven J. Fenves
Health care informatics deals with all the processes or "software" of the health care enterprise: modeling, design, simulation and implementation of health care information systems, support and control of manufacturing and its associated supply chains, and

Demonstration of Rapid Multiplex PCR Amplification Involving 16 Genetic Loci

December 1, 2008
Author(s)
Peter Vallone, Carolyn R. Steffen, John M. Butler
Current forensic DNA typing is conducted in approximately eight to ten hours with steps including DNA extraction, quantitation, polymerase chain reaction (PCR) amplification of multiple short tandem repeat (STR) loci, capillary electrophoresis separation

Addressing Y-Chromosome Short Tandem Repeat(Y-STR) Allele Nomenclature

November 25, 2008
Author(s)
John M. Butler, Margaret C. Kline, Amy E. Decker
Currently 120 different Y-chromosome short tandem repeat (Y-STR) markers are used by various genetic genealogy testing laboratories. In some cases, different laboratories may designate the same Y-STR allele with two different nomenclatures making data
Displaying 576 - 600 of 838