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Health care informatics deals with all the processes or "software" of the health care enterprise: modeling, design, simulation and implementation of health care information systems, support and control of manufacturing and its associated supply chains, and
Leonard F. Pease III, John T. Elliott, De-Hao D. Tsai, Michael R. Zachariah, Michael J. Tarlov
Here we describe the use of electrospray differential mobility analysis (ES-DMA), also known as gas-phase electrophoretic mobility molecular analysis (GEMMA), as a method for measuring low-order aggregates of proteins in solution. We demonstrate proof of
Formamidopyrimidines, 4,6-diamino-5-formamidopyrimidine (FapyAde) and 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua), are among major lesions in DNA generated by hydroxyl radical attack, UV radiation or photosensitization in vitro and in vivo
Current forensic DNA typing is conducted in approximately eight to ten hours with steps including DNA extraction, quantitation, polymerase chain reaction (PCR) amplification of multiple short tandem repeat (STR) loci, capillary electrophoresis separation
Adele P. Peskin, Joe Chalfoun, Alden A. Dima, James J. Filliben
We compare manually segmented masks of cell images that are independently hand-selected by several different people, with a new semi-automated method for estimating reference data for images of cells with very sharp clear edges. By quantifying the large
Currently 120 different Y-chromosome short tandem repeat (Y-STR) markers are used by various genetic genealogy testing laboratories. In some cases, different laboratories may designate the same Y-STR allele with two different nomenclatures making data
J P. Jakupciak, Samantha D. Maragh, Maura E. Markowitz, Alissa K. Greenberg, Mohammad O. Hoque, A Maitra, Peter E. Barker, Paul D. Wagner, William N. Rom, Sudhir Srivastava, David Sidransky, Catherine D. O'Connell
Yadu D. Tewari, Brian E. Lang, Stephen R. Decker, Robert N. Goldberg
Microcalorimetry and high-performance liquid chromatography have been used to conduct a thermodynamic investigation of the following reactions: 1,4--D-xylobiose(aq) + H2O(l) = 2 D-xylose(aq), (1) 1,4--D-xylotriose + 2 H2O(l) = 3 D-xylose(aq), (2) D
Matt S. Munson, John Meacham, David J. Ross, Laurie E. Locascio
A method is described for an aptamer-based affinity assay using a combination of two non-conventional techniques, temperature gradient focusing (TGF) and field amplified continuous sample injection TGF (FACSI-TGF), with fluorescence detection. Human
Mary B. Satterfield, Marc L. Salit, Steven J. Choquette
As a critical component of any microarray experiment, scanner performance has the potential to contribute variability and bias, the magnitude of which is usually not quantified. Using Standard Reference Material (SRM) 2242, certified for Raman spectral
Kinlin L. Chao, Kap Lim, C Lehmann, V Doseeva, A J. Howard, Frederick P. Schwarz, Osnat Herzberg
The crystal structure of YdcF at a resolution of 0.18 nm reveals a fold of the adenine nucleotide α hydrolase-like family. Following the structural clues, a nucleotide binding screen was carried out employing isothermal titration calorimetry. The following
J C. Chiao, Julian M. Goldman, David A. Heck, Peter Kazanzides, William J. Peine, James B. Stiehl, Dwight Yen, Nicholas Dagalakis
With rapid advances in meso, micro and nano scale technology devices and electronics a new generation of advance medical devices is emerging, which promises less invasive and more accurate, automated and effective medical treatments. We examined the
B C. Nelson, Christine M. Pfeiffer, Ming Zhang, David L. Duewer, Katherine E. Sharpless, Katrice Lippa
Background: The National Institute of Standards and Technology (NIST) and the Centers for Disease Control and Prevention (CDC) have recently developed Standard Reference Material (SRM) 1955 Homocysteine and Folate in Frozen Human Serum with certified
Carolyn R. Steffen, Margaret C. Kline, Michael Coble, John M. Butler
An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized to aid in the analysis of degraded DNA samples. These new markers produce short PCR products in the target range of 50 150 base pairs (bp) by moving the
Niya Todorova, V Doseeva, Jayanthi Ramprakash, Frederick P. Schwarz
The binding constant, Kb, and the binding enthalpy (DbHo) of the inhibitor drugs, SB 203580, SKF 86002, and p38 INH.1 binding to two splice variants of p38a MAP kinase and their C162S mutants in 5mM HEPES buffer at pH =7.4 were determined from isothermal
Lili Wang, Adolfas Gaigalas, Gerald Marti, Fatima Abbasi, Robert A. Hoffman
A procedure is presented for calibrating the output of a multicolor flow cytometer in units of antibodies bound per cell (ABC). The procedure involves two steps. First, each of the fluorescence channels of the flow cytometer is calibrated using Ultra
Lili Wang, Kenneth D. Cole, Hua-Jun He, Alexander W. Peterson, Adolfas K. Gaigalas, Y Zong
A recombinant mouse interleukin-4 (IL-4) and three different purified rat anti-mouse IL-4 monoclonal antibodies (Mab) with different epitopes were employed as a model system. This system was used to examine monoclonal antibody effectiveness using both
Robert N. Goldberg, Yadu D. Tewari, Talapady N. Bhat
This review serves to update previously published evaluations of equilibrium constants and enthalpy changes for enzyme catalyzed reactions. For each reaction the following information is given: the reference for the data; the reaction studied; the name of
W Wells, Peter E. Barker, C Macaulay, M Novelli, Richard Levenson, J Crawford
Recent advances in optical imaging technology have the potential to improve the accuracy of disease detection and predict treatment response. The critical end-point for the success of any new imaging technology is clinical outcome. The following review
Dianne L. Poster, John A. Small, Michael T. Postek
The National Nanotechnology Initiative (NNI) in its 2006 document, Environmental, Health, and Safety Research Needs for Engineered Nanoscale Materials (available at www.nano.gov), identifies standards and standard measurement protocols as a critical
Jonghoon Choi, Peter Niarhos, Nam S. Wang, Vytas Reipa
We have covalently attached multiple fluorescent silicon nanocrystals (SNs) to streptavidin molecule. Selective conjugation of SNs to a target protein is accomplished using sequential silicon surface termination chemistry. In the first step, freshly
Understanding the nature of specific neural activity is essential to the progression of research in the field of brain disorders and diseases, as well as neuroprosthetics. Microelectrodes are the primary measurement devices used to transduce neural