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Displaying 1026 - 1050 of 1174

Microsecond time-scale discrimination among polycytidylic acid, polyadenylic acid, and polyuridylic acid as homopolymers or as segments within single RNA molecules

December 1, 1999
Author(s)
M Akeson, D Branton, John J. Kasianowicz, E Brandin, D W. Deamer
Single molecules of DNA or RNA can be detected as they are driven through a single alpha-hemolysin channel by an applied electric field. During translocation, nucleotides within the polynucleotide must pass through the channel pore in sequential single

Challenges in Capturing Oxygenase Activity In Vitro

November 1, 1999
Author(s)
V L. Vilker, Vytautas Reipa, M P. Mayhew, Marcia J. Holden
Biocatalysis using oxygenase or desaturase enzymes has the potential to add value to native fats and oils by adding oxygen hydroxyl groups, or double bonds to create regio- and/or stereospecific products. These enzymes are a subset of the large class of

Heteronuclear NMR and Crystallographic Studies of Wild-Type and H187Q Escherichia Coli Uracil DNA Glycosylase: Electrophilic Catalysis of Uracil Expulsion by a Neutral Histidine 187

September 14, 1999
Author(s)
A C. Drohat, G Xiao, M Tordova, J. Jagadeesh, K Pankiewicz, K A. Watanbe, G L. Gilliland, J T. Stivers
The nature of the putative general acid, His187, in the reaction catalyzed by Escherichia coli uracil DNA glycosylase (UDG) was investigated using X-ray crystallography and NMR spectroscopy. The crystal structures of H187Q UDG, and its complex with uracil

Detection of p53 Gene Mutation: Analysis by Single-Strand Conformation Polymorphism and Cleavase Fragment Length Polymorphism

June 1, 1999
Author(s)
C D. O'Connell, Donald H. Atha, M C. Oldenburg, J Tian, M Siebert, D Handrow, K Grooms, L Heisler, M de Arruda
We have generated a collection of clones containing single point mutations within the exon 5-9 hot spot regions of the p53 gene by using polymerase chain reaction (PCR) to amplify select regions of the gene from characterized cell lines. These clones were

Dynamic Partitioning of Neutral Polymers into a Single Ion Channel

June 1, 1999
Author(s)
S. M. Bezrukov, John J. Kasianowicz
Polymers partitioning into highly confined spaces can be studied using single nanometer-scale pores formed by protein ion channels. The ionic conductance of a channel depends on the state of occupancy of the pore by polymer and serves as a measure of
Displaying 1026 - 1050 of 1174