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Displaying 151 - 175 of 802

Effects of Distal Residues Mutations on the Structure, Dynamics and Catalysis of Human Monoacylglycerol Lipase

January 29, 2018
Author(s)
Sergiy Tyukhtenko, Girija Rajarshi, Ioannis Karageorgos, Nikolai Zvonok, Elyssia S. Gallagher, Hongwei Huang, Kiran Vemuri, Jeffrey W. Hudgens, Alexandros Makriyannis
An understanding of how conformational dynamics modulates function and catalysis of human monoacylglycerol lipase (hMGL), an important pharmaceutical target, can facilitate the development of novel modulatory ligands. Here, we report the discovery and

Recommendations for assessing commutability part 1: general experimental design

January 18, 2018
Author(s)
Johanna Camara, W. Greg Miller, Schimmel Heinz, Neil Greenberg, Chris Burns, Vincent Delatour, Mauro Panteghini, Hubert W. Vesper, Robert Rej, Ferruccio Ceriotti, Gerhard Schumann, Jeffrey R. Budd, Weykamp Cas, Goran Nilsson, Finlay MacKenzie, Thomas Keller
Commutability is a property of a reference material that relates to the closeness of agreement between results for a reference material and results for clinical samples when measured by two or more measurement procedures. Commutability of reference

Recommendations for assessing commutability part 2: based on the difference in bias between a reference material and clinical samples

January 18, 2018
Author(s)
Johanna Camara, Goran Nilsson, Jeffrey R. Budd, Neil Greenberg, Vincent Delatour, Gerhard Schumann, Robert Rej, Mauro Panteghini, Ferruccio Ceriotti, Schimmel Heinz, Weykamp Cas, Thomas Keller, Chris Burns, Finlay MacKenzie, W. Greg Miller
A process is described to assess the commutability of a reference material intended for use as a calibrator, trueness control or external quality assessment sample based on the difference in bias between a reference material and clinical samples measured

Recommendations for assessing commutability part 3: based on the calibration effectiveness of a reference material

January 18, 2018
Author(s)
Johanna Camara, Weykamp Cas, Goran Nilsson, Robert Rej, Finlay MacKenzie, Ferruccio Ceriotti, Neil Greenberg, Schimmel Heinz, Gerhard Schumann, Thomas Keller, Vincent Delatour, Mauro Panteghini, Chris Burns, W. Greg Miller
A process is described to assess the commutability of a reference material intended for use as a calibrator based on its ability to fulfill its intended use in a calibration traceability scheme to produce equivalent clinical sample results among different

Quantitative analysis of the impact of a human pathogenic mutation on the CCT5 chaperonin subunit using a proxy archaeal ortholog

December 1, 2017
Author(s)
Darion Spigolon, David Travis Gallagher, Adrian Velazquez-Campoy, Donatella Bulone, Jatin Narang, Pier San Biagio, Francesco Cappello, Alberto J. Macario, Everly Conway de Macario, Frank Robb
The human chaperonin complex is a 1 MDa nanomachine composed of two octameric rings formed from eight similar but non-identical subunits called CCT. Here, we are elucidating the mechanism of a heritable CCT5 subunit mutation that causes profound neuropathy

Tissue Inhibitor of Metalloprotease-2: bioprocess development, physicochemical, biochemical and biological characterization of highly expressed recombinant protein

December 1, 2017
Author(s)
Ananda Chowdhury, Robert G. Brinson, Beiyang Wei, William G. Stetler-Stevenson
Tissue Inhibitor of Metalloprotease -2 (TIMP-2) is a secreted, 21 kDa, multifunctional protein. It was first described as an endogenous inhibitor of matrix metalloproteases (MMPs) that prevents protease-mediated breakdown of the extracellular matrix often

Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

November 20, 2017
Author(s)
Lili Wang, Adolfas K. Gaigalas, James Wood
Multicolor flow cytometer assays are routinely used in clinical laboratories for immunophenotyping, monitoring disease and treatment, and determining prognostic factors. However, existing methods for quantitative measurements have not yet produced

Phase noise generated by vibration of bacteria adhered to a quartz resonator

September 22, 2017
Author(s)
Ward L. Johnson, Danielle C. France, F L. Walls, William T. Cordell, Nikki S. Rentz
An approach is presented for sensing mechanical fluctuations of bacteria, with potential application in monitoring microbial responses to changes in chemical environment, such as antimicrobial intervention. Bacteria are tethered to a quartz crystal

Using metagenomic methods to detect organismal contaminants in microbial materials.

September 12, 2017
Author(s)
Nathanael D. Olson, Justin M. Zook, Jayne B. Morrow, Nancy J. Lin
High sensitivity methods as next generation sequencing and PCR are adversely impacted by organismal and DNA contaminants. Current methods for detecting contaminants in microbial materials (genomic DNA and cultures) are not sensitive enough and require

Small molecule inhibitors of DNA glycosylases as potential drugs in cancer therapy

August 31, 2017
Author(s)
M Miral Dizdar, Aaron C. Jacobs, Nathan Donley, Marcus J. Calkins, Ajit Jadhav, Dorjbal Dorjsuren, David Maloney, Anton Simeonov, Pawel Jaruga, Erdem Coskun, Amanda K. McCullough, R. S. Lloyd
Statement of the Problem: Most chemotherapeutic agents kill cancer cells by damaging DNA. Cancer cells overexpress DNA repair proteins and thus increase DNA repair capacity that can cause resistance to therapy by removing DNA lesions before they become

A serum small molecule biosignature of radiation exposure from total body irradiated patients

August 21, 2017
Author(s)
Evagelia C. Laiakis, Evan L. Pannkuk, Siddheshwar Chauthe, Yi-Wen Wang, Ming Liang, Tytus Mak, Christopher A. Barker, Giuseppe Astarita, Albert J. Fornace
The potential of radiological accidents and nuclear terrorism have increased the need for development of new rapid biodosimetry methods. In addition, in a clinical setting the issue of an individual's radiosensitivity should be taken into consideration

Genome-wide reconstruction of complex structural variants using read clouds

July 17, 2017
Author(s)
Noah Spies, Ziming Weng, Alex Bishara, Jennifer H. McDaniel, David N. Catoe, Justin M. Zook, Marc L. Salit, Robert B. West, Serafim Batzoglou, Arend Sidow
Recently developed methods that utilize partitioning of long genomic DNA fragments, and barcoding of shorter fragments derived from them, have succeeded in retaining long-range information in short sequencing reads. These so-called read cloud approaches

FLOW CYTOMETER PERFORMANCE CHARACTERIZATION, STANDARDIZATION AND CONTROL

May 9, 2017
Author(s)
Lili Wang, Robert A. Hoffman
Flow cytometry is a widely used technique for the analysis of single cells and particles. It is an essential tool for immunological research, drug and device development, clinical trials, disease diagnosis, and therapy monitoring. However, the measurements

Conformational Changes in Active and Inactive States of Human PP2C? characterized by Hydrogen/Deuterium Exchange-Mass Spectrometry

May 8, 2017
Author(s)
Sharlyn Mazur, Elyssia S. Gallagher, Subrata Debnath, Stewart R. Durell, Kyle Anderson, Lisa M. Jenkins, Ettore Appella, Jeffrey W. Hudgens
PPM serine/threonine protein phosphatases function in signaling pathways and require millimolar concentrations of Mn2+ or Mg2+ ions for activity. Whereas the crystal structure of human PP2Cα displayed two tightly bound Mn2+ ions in the active site, recent
Displaying 151 - 175 of 802