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Displaying 226 - 250 of 1174

Workshop 9: Control Cells or Not

June 17, 2019
Author(s)
Paul Wallace, Jonni S. Moore, Derek Jones, Litwin Virginia, Lili Wang, Yanli Liu
"Control Cells or Not" was an educational workshop that used surveys, lectures, and discussions to identify problems and solutions related to using commercially available control cell products and lab-developed approaches. Collective efforts are proposed

High-coverage, long-read sequencing of Chinese trio reference samples

June 14, 2019
Author(s)
Justin M. Zook, Nathanael D. Olson, Marc L. Salit, Aaron Wenger, Chunlin Xiao, Robert Sebra
Genome In a Bottle (GIAB) is a consortium hosted by the National Institute of Standards and Technology whose primary objective is the development and characterization of human genomic reference materials. The consortium includes representatives from

Structure and Function in Antimicrobial Piscidins: Histidine Position, Directionality of Membrane Insertion, and pH-Dependent Permeabilization

May 30, 2019
Author(s)
Mihaela Mihailescu, Mirco Sorci, Jolita Seckute, Vitalii I. Silin, Janet Hammer, B. Scott Perrin, Jorge Hernandez, Nedzada Smajic, Akritee Shrestha, Kimberly Bogadardus, Alexander Greenwood, Riqiang Fu, Jack Blazyk, Richard W. Pastor, Linda Nicholson, Georges Belfort, Myriam Cotten
Piscidins are histidine-enriched antimicrobial peptides that interact with lipid bilayers as amphipathic alpha-helices. Their activity at acidic and basic pH in vivo makes them promising templates for biomedical applications. This study focuses on p1 and

Structural Insights into DNA-Stabilized Silver Clusters

May 10, 2019
Author(s)
Danielle Schultz, Robert G. Brinson, Fahriye N. Sari, Jeffrey Fagan, Christina Bergonzo, Nancy Lin, Joy Dunkers
The structure and dynamics of Ag complexes derived from single stranded DNA (ssDNA) is less understood than their double stranded (dsDNA) counterparts despite their great promise as fluorescent biological probes and sensors. In this work, we seek new

Strategies for robust and accurate measurement of nanomaterial bioaccumulation

April 17, 2019
Author(s)
Elijah J. Petersen, Monika Mortimer, Robert Burgess, Richard Handy, Shannon Hanna, Kay Ho, Monique E. Johnson, Susana Louriero, Selck Henriette, Janeck Scott-Fordsmand, David Spurgeon, Jason Unrine, Nico van den Brink, Ying Wang, Jason White, Patricia Holden
One of the key components for environmental risk assessment of engineered nanomaterials (ENMs) is data on bioaccumulation potential. Accurately measuring bioaccumulation can be critical for regulatory decision making regarding material hazard and risk, and

Ligand-based stability changes in duplex DNA measured with a microscale electrochemical platform

April 12, 2019
Author(s)
Sarah M. Robinson, Zuliang Shen, Jon R. Askim, Christopher B. Montgomery, Herman O. Sintim, Stephen Semancik
Development of technologies for rapidly screening the thermal stability of DNA secondary structures and the effects on stability for binding of small molecule drugs is important to the drug discovery process. In this report, we describe the capabilities of

An open resource for accurately benchmarking small variant and reference calls

April 1, 2019
Author(s)
Justin M. Zook, Jennifer H. McDaniel, Marc L. Salit, Nathanael D. Olson, Justin M. Wagner
Benchmark small variant calls are required for developing, optimizing and assessing the performance of sequencing and bioinformatics methods. Here, as part of the Genome in a Bottle (GIAB) Consortium, we apply a reproducible, cloud-based pipeline to

Best practices for benchmarking germline small-variant calls in human genomes

March 11, 2019
Author(s)
Justin M. Zook, Marc L. Salit
Standardized benchmarking approaches are required to assess the accuracy of variants called from sequence data. Although variant- calling tools and the metrics used to assess their performance continue to improve, important challenges remain. Here, as part

Engineering ClpS for Selective and Enhanced N-terminal Amino Acid Binding

March 1, 2019
Author(s)
Jennifer A. Tullman, Zvi Kelman, John P. Marino
One of the central challenges in the development of single-molecule protein sequencing technologies is achieving high-fidelity, sequential recognition and detection of specific amino acids that comprise the peptide sequence. An approach towards achieving

Comparison of volumetric and bead-based counting of CD34 cells by single-platform flow cytometry

February 20, 2019
Author(s)
Luisa Saraiva, Lili Wang, Martin Kammel, A. Kummrow, E Atkinson, J Y. Lee, B Yalcinkaya, M Akgoz, A Ruf, A Engel, Yu-Zhong Zhang, O O''Shea, M P. Sassi, C Divieto, T Lekishvili, J J. Campbell, Y Liu, J Wang, R Stebbings, Adolfas Gaigalas, P Rigsby, J Neukammer, S Vessillier
Background: Over 2000 people a year in the UK need a bone marrow or blood stem cell transplant. It is important to accurately quantify the haematopoietic stem cells to predict whether the transplant will be successful in replenishing the immune system

Proceedings of the First Workshop on Standards for Microfluidics

January 28, 2019
Author(s)
Darwin R. Reyes-Hernandez, Henne van Heeren
In the last two decades, the microfluidics/lab-on-a-chip field has evolved from the concept of micro total analysis systems, where systems with integrated pretreatment and analysis of chemicals were envisioned, to what is known today as lab-on-a-chip

CrowdVariant: a crowdsourcing approach to curate copy number variants

January 6, 2019
Author(s)
Justin M. Zook, Marc L. Salit, Peyton Greenside, Ryan Poplin, Mark DePristo, Madeleine Cule
Copy number variants (CNVs) are an important type of genetic variation and play a causal role in many diseases. However, they are also notoriously difficult to identify accurately from next-generation sequencing (NGS) data. For larger CNVs, genotyping

Cooperative Dynamics in a Model DPPC Membrane Arise from Membrane Layer Interactions

December 19, 2018
Author(s)
Kiley Kennedy, Neha Shafique, Jack F. Douglas, Francis W. Starr
The dynamics of model membranes can be highly heterogeneous, especially in more ordered dense phases. Experimentally, monolayers are often studied as a model system to infer the properties of membranes. To better understand the origins of this

Innovative Approaches to Combat Healthcare-Associated Infections Using Efficacy Standards Developed Through Industry and Federal Collaboration

October 5, 2018
Author(s)
Dianne L. Poster, Carl C. Miller, Yaw S. Obeng, Michael T. Postek, Troy E. Cowan, Richard A. Martinello
Nation-wide, healthcare-associated infections (HAIs) infect one in every 25 hospital patients, account for more than 100,000 deaths and increase medical costs by around $96-147B, each year. Ultraviolet-C (UV-C) antimicrobial devices are shown to reduce the

Combinatorial Cassettes for Higher-Throughput Screening of Osteogenesis

October 4, 2018
Author(s)
Carl G. Simon Jr., Subhadip Bodhak, Hariharan K. Iyer, Luis Fernandez de Castro Diaz, Pam Robey, Azusa Maeda, Sergei Kuznetsov
Animal models are the most biologically relevant method for measuring the osteogenic capability of bone graft formulations. However, animal experiments are slow, tedious and the results can be highly variable, even when conducted within the same lab. In

Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli

October 1, 2018
Author(s)
Prasad T. Reddy, Robert G. Brinson, James T. Hoopes, Colleen McClung, Na Ke, Lila Kashi, Mehmet Berkmen
Proteins labeled with stable isotopes play an important role in structural and biophysical studies including nuclear magnetic resonance, small angle neutron scattering, neutron reflectometry, quantitative mass spec and more. The most common and cost

Platform development for expression and purification of stable isotope labeled monoclonal antibodies in Escherichia coli

October 1, 2018
Author(s)
Robert Brinson, James T. Hoopes, Colleen McClung, Na Ke, Lila Kashi, Mehmet Berkmen, Zvi Kelman, Prasad T. Reddy
Proteins labeled with stable isotopes play an important role in structural and biophysical studies including nuclear magnetic resonance, small angle neutron scattering, neutron reflectometry, quantitative mass spec and more. The most common and cost
Displaying 226 - 250 of 1174