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Displaying 76 - 100 of 239

Combined Effects of High-Dose Bisphenol A and Oxidizing Agent (KBrO3) on Cellular Microenvironment, Gene Expression, and Chromatin Structure of Ku70-deficient Mouse Embryonic Fibroblasts

April 15, 2016
Author(s)
Erdem Coskun, Pawel Jaruga, Miral M. Dizdar, Natalie Gassman, Samuel Wilson
Background: Exposure to the environmental endocrine disrupting chemical bisphenol A (BPA) has been reported to alter global gene expression, induce epigenetic modifications, and interfere with the complex regulatory networks of cells. In addition to these

Inhibitors of DNA glycosylases as potential therapeutic drugs in cancer

April 4, 2016
Author(s)
M Miral Dizdar, Aaron C. Jacobs, Nathan Donley, Ajit Jadhav, Dorjbal Dorjsuren, David Maloney, Anton Simeonov, Pawel Jaruga, Erdem Coskun, Amanda K. McCullough, R. S. Lloyd
Ionizing radiation and most chemotherapeutic agents kill tumor cells by damaging DNA. The efficacy of DNA-damaging agents may be influenced by increased DNA repair capacity in tumors that results from overexpression of DNA repair proteins. Inhibition of

SMALL MOLECULE INHIBITORS OF DNA GLYCOSYLASES AS POTENTIAL DRUGS IN CHEMO- AND RADIOTHERAPIES

March 19, 2016
Author(s)
M Miral Dizdar, Aaron C. Jacobs, Nathan Donley, Marcus J. Calkins, Ajit Jadhav, Dorjbal Dorjsuren, David Maloney, Anton Simeonov, Amanda K. McCullough, R S. Lloyd, Erdem Coskun, Pawel Jaruga
Introduction: Ionizing radiation and most chemotherapeutic agents kill tumor cells by damag-ing DNA. The efficacy of DNA-damaging agents may be influenced by increased DNA repair capacity in tumors that results from overexpression of DNA repair proteins

GC-MS/MS Measurement of Nanomaterial Induced Genotoxicity in Isolated DNA

November 24, 2015
Author(s)
Bryant C. Nelson, Elijah J. Petersen, Pawel Jaruga, M Miral Dizdar
Making accurate measurements of the environmental fate and environmental and biological effects of engineered nanomaterials (ENMs) is critical for reliable risk assessment of these materials. ENM induced oxidative stress and/or direct binding of ENMs to

GC/MS Measurement of Nanomaterial Induced Genotoxicity in Isolated DNA

November 24, 2015
Author(s)
Bryant C. Nelson, Elijah J. Petersen, Pawel Jaruga, M Miral Dizdar
Making accurate measurements of the environmental fate and environmental and biological effects of engineered nanomaterials (ENMs) is critical for reliable risk assessment of these materials. ENM induced oxidative stress and/or direct binding of ENMs to

LC-MS/MS Measurement of Nanomaterial Induced Genotoxicity in Isolated DNA

November 24, 2015
Author(s)
Bryant C. Nelson, Elijah J. Petersen, Pawel Jaruga, M Miral Dizdar
Making accurate measurements of the environmental fate and environmental and biological effects of engineered nanomaterials (ENMs) is critical for reliable risk assessment of these materials. However, the unique behaviors of ENMs may cause artifacts in

MEASUREMENT OF DNA REPAIR PROTEINS IN RELATION TO DISEASE BIOMARKERS AND DRUG DEVELOPMENT

November 7, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Leona D. Scanlan, Alessandro Tona, Mark S. Lowenthal, Prasad T. Reddy, M Miral Dizdar, Ann-Sofie Jemth, Olga Loseva, Thomas Helleday
Introduction: In aerobic organisms, intracellular metabolism and exogenous sources such as ionizing radiation and carcinogenic compounds generate reactive species including free radicals derived from either oxygen or nitrogen. Oxidative stress thereby

DNA Extraction and DNA Damage Measurement in the Nematode Caenorhabditis elegans

November 1, 2015
Author(s)
Leona D. Scanlan, Pawel Jaruga, Sanem Hosbas Coskun, Jamie L. Almeida, David N. Catoe, Jennifer McDaniel, Miral M. Dizdar
Little is known about endogenous DNA damage in the nematode. In this work, we standardized the growth of the nematode in two different growth media (axenic CeHR supplemented with 20% milk and S-basal with E. coli), and developed a novel high-salt, phenol

Extreme expression of DNA repair protein apurinic/apyrimi-dinic endonuclease I (APE1) in human breast cancer as measured by liquid-chromatography isotope-dilution tandem mass spectrometry

September 15, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Prasad T. Reddy, Miral M. Dizdar
Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA re-pair protein and plays other important roles. Increased lev-els of APE1 in cancer have been reported. However, avail-able methods to measure APE1 levels are indirect and not quantitative. We

Extreme Expressions of DNA Repair Proteins APE1 and MTH1, in Human Breast Cancer as Measured by Liquid Chromatography and Isotope Dilution Tandem Mass Spectrometry

September 15, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Leona D. Scanlan, Alessandro Tona, Mark S. Lowenthal, Prasad T. Reddy, M Miral Dizdar, Ann-Sofie Jemth, Olga Loseva, Thomas Helleday
Accurate measurement of DNA repair proteins in cancer tissues is becoming more important due to the individual and origin based expression differences in cancer patients as well as the novel approach of using the repair enzyme inhibitors in cancer

Small Molecule Inhibitors of 8-Oxoguanine DNA Glycosylase-1 (OGG1)

August 7, 2015
Author(s)
Pawel Jaruga, Erdem Coskun, M Miral Dizdar, Nathan Donley, Stephen Lloyd, Amanda McCullough
ABSTRACT The DNA base excision repair (BER) pathway, which utilizes DNA glycosylases to initiate repair of specific DNA lesions, is the major pathway for the repair of DNA damage induced by oxidation, alkylation, and deamination. Early results from

Production, Purification and Characterization of 15N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric Measurements

July 26, 2015
Author(s)
Prasad T. Reddy, Pawel Jaruga, Bryant C. Nelson, Mark Lowenthal, Ann-Sofie Jemth, Olga Loseva, Erdem Coskun, Thomas Helleday, Miral M. Dizdar
Oxidatively induced DNA damage is caused in living organisms by a variety of damaging agents, resulting in the formation of a multiplicity of lesions, which are mutagenic and cytotoxic. Unless repaired by DNA repair mechanisms before DNA replication, DNA

Addiction to MTH1 protein results in intense expression in human breast cancer tissue as measured by liquid chromatography-isotope-dilution tandem mass spectrometry

June 23, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Ann-Sofie Jemth, Olga Loseva, Leona D. Scanlan, Alessandro Tona, Mark S. Lowenthal, Thomas Helleday, M Miral Dizdar
MTH1 protein sanitizes the nucleotide pool so that modified 2'-deoxynucleoside triphosphates (dNTPs) cannot be used in DNA replication. Cancer cells require MTH1 to avoid incorporation of modified dNTPs resulting in DNA damage mutations and cell death