September 1, 2011
Author(s)
Marc L. Salit, Lichun Jiang, Felix Schlesinger, Brian Oliver, Carrie Davis, Yu Zhang, Renhua Li, Thomas R. Gingeras
High-throughput sequencing of cDNA (RNA-seq) is a widely deployed transcriptome profiling and annotation technique, but questions about the performance of different protocols and platforms remain. We used a newly developed pool of 96 synthetic RNAs with