U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Search Publications by: Lili Wang (Fed)

Search Title, Abstract, Conference, Citation, Keyword or Author
Displaying 26 - 50 of 157

Comparison of volumetric and bead-based counting of CD34 cells by single-platform flow cytometry

February 20, 2019
Author(s)
Luisa Saraiva, Lili Wang, Martin Kammel, A. Kummrow, E Atkinson, J Y. Lee, B Yalcinkaya, M Akgoz, A Ruf, A Engel, Yu-Zhong Zhang, O O''Shea, M P. Sassi, C Divieto, T Lekishvili, J J. Campbell, Y Liu, J Wang, R Stebbings, Adolfas Gaigalas, P Rigsby, J Neukammer, S Vessillier
Background: Over 2000 people a year in the UK need a bone marrow or blood stem cell transplant. It is important to accurately quantify the haematopoietic stem cells to predict whether the transplant will be successful in replenishing the immune system

Methodology for evaluating and comparing fluorescence measurement capabilities: Multi-site study of 23 flow cytometers

September 23, 2018
Author(s)
David R. Parks, Wayne A. Moore, Ryan Brinkman, Yong Chen, Danilo Condello, Faysal E. Khettabi, John P. Nolan, Stephen P. Perfetto, Doug Redelman, Josef Spidlen, Jonathan V. Dyke, Lili Wang, James C. Wood
We developed measurement methods and analysis procedures to perform inter-instrument comparisons and used them to evaluate and compare the fluorescence measurement capabilities among 23 cytometers in 9 laboratories selected to provide a range of

Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

November 20, 2017
Author(s)
Lili Wang, Adolfas K. Gaigalas, James Wood
Multicolor flow cytometer assays are routinely used in clinical laboratories for immunophenotyping, monitoring disease and treatment, and determining prognostic factors. However, existing methods for quantitative measurements have not yet produced

FLOW CYTOMETER PERFORMANCE CHARACTERIZATION, STANDARDIZATION AND CONTROL

May 9, 2017
Author(s)
Lili Wang, Robert A. Hoffman
Flow cytometry is a widely used technique for the analysis of single cells and particles. It is an essential tool for immunological research, drug and device development, clinical trials, disease diagnosis, and therapy monitoring. However, the measurements

Standardization, Calibration, and Control in Flow Cytometry

January 6, 2017
Author(s)
Lili Wang, Robert A. Hoffman
Standardization, control, and calibration provide different degrees of certainty about the data acquired with an instrument. Each process is aimed at assuring that results from the instrument have the quality required for the intended purpose. The purpose

Quantitative Flow Cytometry Measurements in Antibody Bound per Cell Based on CD4 Reference

February 12, 2016
Author(s)
Lili Wang, Heba Degheidy, Fatima Abbasi, Howard Mostowski, Gerald Marti, Steven R. Bauer, Robert Hoffman, Adolfas K. Gaigalas
Multicolor flow cytometer assays are with fluorescently labeled antibodies routinely used in clinical laboratories to measure the cell number of specific immunophenotypes and to estimate expression levels of specific receptors/antigens either on the cell

Quantification of Cells with Specific Phenotypes I: Determination of CD4+ Cell Count per mL in Reconstituted Lyophilized Human PBMC Pre-labelled with Anti-CD4 FITC Antibody

March 10, 2015
Author(s)
Richard Stebbings, Lili Wang, Janet Sutherland, Martin Kammel, Adolfas Gaigalas, M. John, B. Roemer, M. Kuhne, R. J. Schneider, M. Braun, N. Leclere, D. Dikshit, Fatima Abbasi, Gerald Marti, P. Porcedda, M. Sassi, L. Revel, S. K. Kim, D. Marshall, L. Whitby, Jing Wang, V. Ost, M. Vonski, Joerg Neukammer
A surface-labelled lyophilised lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells (PBMC) pre-labelled with a fluorescein isothiocyanate (FITC) conjugated anti-CD4 monoclonal antibody. The sLL preparation is