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Search Publications by: Lili Wang (Fed)

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Displaying 1 - 25 of 73

Standards and Metrology for Viral Vectors as Molecular Tools: Outcomes from a CCQM Workshop

May 24, 2024
Author(s)
Janathan Campbell, Neil Almond, Y Bae, Ravneet Bhuller, Andrea Briones, S-Y CHO, Megan Cleveland, Thomas Cleveland, Francis Galaway, Hua-Jun He, U Herbrand, Jim Huggett, Sarah Kempster, Ibolya Kepiro, Afifa Khan, Edward Kwee, Wilson Li, Sheng Lin-Gibson, Luise Luckau, Caterina Minelli, M Ryadnov, I Searing, Lili Wang, Alexandra Whale, Julian Braybrook
Viral vectors are agents enabling gene transfer and genome editing and have widespread utility across the healthcare and biotechnology sectors. In January 2023, the International Bureau for Weights and Measures' Consultative Committee for Amount of

Size and Number Concentration Measurements of Polystyrene Nanoparticle Suspensions using Several Different Techniques

September 8, 2022
Author(s)
Paul C. DeRose, Kurt D. Benkstein, Elzafir B. Elsheikh, Adolfas K. Gaigalas, Sean Lehman, Dean C. Ripple, Linhua Tian, Wyatt N. Vreeland, Adam York, Yu-Zhong Zhang, Lili Wang
The number concentrations of nominal 100 nm, 200 nm and 500 nm diameter, fluorescently-labeled polystyrene nanosphere suspensions were measured using seven different techniques. Diameter values were also measured where possible. The diameter values were

Expanding NIST’s Calibration of Fluorescent Microspheres for Flow Cytometry to More Fluorescence Channels and Smaller Particles

September 16, 2020
Author(s)
Paul C. DeRose, Linhua Tian, Elzafir B. Elsheikh, Aaron A. Urbas, Yu-Zhong Zhang, Lili Wang
NIST, NIH and other industry stakeholders have been working together to enable fluorescence intensities of flow cytometer calibration beads to be assigned quantitative “ERF” values with high accuracy and precision. The ultimate goal of this effort is to

Measurement and Standardization Challenges for Exosome-Based Delivery Vectors

September 4, 2020
Author(s)
Bryant C. Nelson, Lili Wang, Samantha D. Maragh, Paul C. DeRose, Elzafir B. Elsheikh, Wyatt N. Vreeland, Ionita Ghiran, Jennifer Jones
Extracellular vesicles (EVs), and in particular exosomes, have the potential to revolutionize the development and efficient delivery of clinical therapeutics. In this Perspective, we focus on providing a brief introduction to the landscape of exosome-based

An accurate and rapid single step protocol for enumeration of cytokine positive T lymphocytes

September 3, 2020
Author(s)
Deepa Rajagopal, Linhua Tian, Shiqiu Xiong, Lili Wang, Jonathan Campbell, Luisa Saraiva, S Vessillier
Activation of T lymphocytes leads to differentiation, characterized by the ability to induce production of cytokines. Accurate determination of cellular subsets that secrete particular cytokine(s) is therefore, a significant parameter for functional

Workshop 13: Building Measurement Assurance in Flow Cytometry

June 17, 2019
Author(s)
Lili Wang, Stephen Perfetto, Robert Hoffman, John T. Elliott, Sheng Lin-Gibson, Steven Bauer, Heba Degheidy, Judith Arcidiacono, Litwin Virginia
Two workshops were held to identify measurement challenges and potential solutions for building measurement assurance for flow cytometry. This report summarizes key findings, including the need for high quality reagents, reference standards or materials

Workshop 9: Control Cells or Not

June 17, 2019
Author(s)
Paul Wallace, Jonni S. Moore, Derek Jones, Litwin Virginia, Lili Wang, Yanli Liu
"Control Cells or Not" was an educational workshop that used surveys, lectures, and discussions to identify problems and solutions related to using commercially available control cell products and lab-developed approaches. Collective efforts are proposed

Comparison of volumetric and bead-based counting of CD34 cells by single-platform flow cytometry

February 20, 2019
Author(s)
Luisa Saraiva, Lili Wang, Martin Kammel, A. Kummrow, E Atkinson, J Y. Lee, B Yalcinkaya, M Akgoz, A Ruf, A Engel, Yu-Zhong Zhang, O O''Shea, M P. Sassi, C Divieto, T Lekishvili, J J. Campbell, Y Liu, J Wang, R Stebbings, Adolfas Gaigalas, P Rigsby, J Neukammer, S Vessillier
Background: Over 2000 people a year in the UK need a bone marrow or blood stem cell transplant. It is important to accurately quantify the haematopoietic stem cells to predict whether the transplant will be successful in replenishing the immune system

Methodology for evaluating and comparing fluorescence measurement capabilities: Multi-site study of 23 flow cytometers

September 23, 2018
Author(s)
David R. Parks, Wayne A. Moore, Ryan Brinkman, Yong Chen, Danilo Condello, Faysal E. Khettabi, John P. Nolan, Stephen P. Perfetto, Doug Redelman, Josef Spidlen, Jonathan V. Dyke, Lili Wang, James C. Wood
We developed measurement methods and analysis procedures to perform inter-instrument comparisons and used them to evaluate and compare the fluorescence measurement capabilities among 23 cytometers in 9 laboratories selected to provide a range of

Quantitative Fluorescence Measurements with Multicolor Flow Cytometry

November 20, 2017
Author(s)
Lili Wang, Adolfas K. Gaigalas, James Wood
Multicolor flow cytometer assays are routinely used in clinical laboratories for immunophenotyping, monitoring disease and treatment, and determining prognostic factors. However, existing methods for quantitative measurements have not yet produced

FLOW CYTOMETER PERFORMANCE CHARACTERIZATION, STANDARDIZATION AND CONTROL

May 9, 2017
Author(s)
Lili Wang, Robert A. Hoffman
Flow cytometry is a widely used technique for the analysis of single cells and particles. It is an essential tool for immunological research, drug and device development, clinical trials, disease diagnosis, and therapy monitoring. However, the measurements

Standardization, Calibration, and Control in Flow Cytometry

January 6, 2017
Author(s)
Lili Wang, Robert A. Hoffman
Standardization, control, and calibration provide different degrees of certainty about the data acquired with an instrument. Each process is aimed at assuring that results from the instrument have the quality required for the intended purpose. The purpose

Quantitative Flow Cytometry Measurements in Antibody Bound per Cell Based on CD4 Reference

February 12, 2016
Author(s)
Lili Wang, Heba Degheidy, Fatima Abbasi, Howard Mostowski, Gerald Marti, Steven R. Bauer, Robert Hoffman, Adolfas K. Gaigalas
Multicolor flow cytometer assays are with fluorescently labeled antibodies routinely used in clinical laboratories to measure the cell number of specific immunophenotypes and to estimate expression levels of specific receptors/antigens either on the cell

Quantification of Cells with Specific Phenotypes I: Determination of CD4+ Cell Count per mL in Reconstituted Lyophilized Human PBMC Pre-labelled with Anti-CD4 FITC Antibody

March 10, 2015
Author(s)
Richard Stebbings, Lili Wang, Janet Sutherland, Martin Kammel, Adolfas Gaigalas, M. John, B. Roemer, M. Kuhne, R. J. Schneider, M. Braun, N. Leclere, D. Dikshit, Fatima Abbasi, Gerald Marti, P. Porcedda, M. Sassi, L. Revel, S. K. Kim, D. Marshall, L. Whitby, Jing Wang, V. Ost, M. Vonski, Joerg Neukammer
A surface-labelled lyophilised lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells (PBMC) pre-labelled with a fluorescein isothiocyanate (FITC) conjugated anti-CD4 monoclonal antibody. The sLL preparation is

Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry

December 11, 2014
Author(s)
Meiyao M. Wang, Martin Misakian, Hua-Jun He, Peter Bajcsy, Jeffrey M. Davis, Kenneth D. Cole, Illarion Turko, Lili Wang, Fatima Abbasi
For quantitative flow cytometry, a biological cell reference material with a known biomarker expression level is needed to transform a linear arbitrary fluorescence intensity scale obtained with fluorescent microspheres to an antibody bound per cell (ABC)

Flow Cytometer Performance Characterization, Standardization and Calibration against CD4 on T Lymphocytes Enables Quantification of Biomarker Expressions for Immunological Applications

August 7, 2014
Author(s)
Heba Degheidy, Steven R. Bauer, Gerald Marti, Lili Wang
There is an urgent need for developing a procedure for biomarker standardization and quantification in clinical laboratories. Measuring the expression levels of cell antigens is critical for the diagnosis of many diseases, e.g. leukemia, lymphoma and

Breast Cancer Biomarker Measurements and Standards

January 24, 2013
Author(s)
Kenneth D. Cole, Lili Wang, Hua-Jun He
Cancer is a heterogeneous disease characterized by changes in the levels and activities of important cellular proteins, including oncogenes and tumor suppressors. Genetic mutations cause changes in protein activity and protein expression levels that result