Publications

Displaying 51 - 75 of 157

Measurement of Microsphere Concentration Using a Flow Cytometer with Volumetric Sample Delivery

December 29, 2014

Author(s)

Lili Wang, Steven J. Choquette, Yu-Zhong Zhang

Microsphere concentrations are needed to assign ERF units to microspheres used in quantitative flow cytometry. An Attune® acoustic focusing flow cytometer was evaluated for the measurement of the concentration of microspheres contained in a Trucount® (TC)

Quantifying CD4 receptor protein in two human CD4+ lymphocyte preparations for quantitative flow cytometry

December 11, 2014

Author(s)

Meiyao M. Wang, Martin Misakian, Hua-Jun He, Peter Bajcsy, Jeffrey M. Davis, Kenneth D. Cole, Illarion Turko, Lili Wang, Fatima Abbasi

For quantitative flow cytometry, a biological cell reference material with a known biomarker expression level is needed to transform a linear arbitrary fluorescence intensity scale obtained with fluorescent microspheres to an antibody bound per cell (ABC)

Flow Cytometer Performance Characterization, Standardization and Calibration against CD4 on T Lymphocytes Enables Quantification of Biomarker Expressions for Immunological Applications

August 7, 2014

Author(s)

Heba Degheidy, Steven R. Bauer, Gerald Marti, Lili Wang

There is an urgent need for developing a procedure for biomarker standardization and quantification in clinical laboratories. Measuring the expression levels of cell antigens is critical for the diagnosis of many diseases, e.g. leukemia, lymphoma and

Breast Cancer Biomarker Measurements and Standards

January 24, 2013

Author(s)

Kenneth D. Cole, Lili Wang, Hua-Jun He

Cancer is a heterogeneous disease characterized by changes in the levels and activities of important cellular proteins, including oncogenes and tumor suppressors. Genetic mutations cause changes in protein activity and protein expression levels that result

Measurement of Scattering and Absorption Cross Sections of Microspheres for Wavelengths between 240 nm and 800 nm

January 10, 2013

Author(s)

Lili Wang, Steven J. Choquette, Adolfas Gaigalas

A commercial spectrometer with a 150 mm integrating sphere (IS) detector was used to measure the scattering and absorption cross sections of polystyrene monodisperse microspheres suspended in water. Absorption measurements were performed with the sample

Quantifying CD4 Receptor Density on Human T Lymphocytes Using Multiple Reaction Monitoring Mass Spectrometry

January 3, 2013

Author(s)

Meiyao M. Wang, Hua-Jun He, Illarion Turko, Karen W. Phinney, Lili Wang

CD4 is an important glycoprotein containing a transmembrane portion and a short intracellular tail. It locates on the surface of various types of immune cells and performs critical role in multiple cellular functions such as signal amplification and

Measurement of Scattering Cross Section with a Spectrophotometer with an Integrating Sphere Detector

September 13, 2012

Author(s)

Lili Wang, Steven J. Choquette, Yu-Zhong Zhang, Victoria Karpiak, Adolfas Gaigalas

A commercial spectrometer with an integrating sphere (IS) detector was used to measure the scattering cross section of microspheres. Analysis of the measurement process showed that two measurements of the extinction, one with the cuvette placed in the

NIST/ISAC standardization study: variability in assignment of intensity values to fluorescence standard beads and in cross calibration of standard beads to hard dyed beads

August 28, 2012

Author(s)

Lili Wang

A procedure is described for assigning the number of equivalent reference fluorophores (ERF) values to microspheres labeled with a fluorophore designed to produce a fluorescence response in a given fluorescence channel of a multicolor flow cytometer. The

Human CD4+ Lymphocytes for Antigen Quantification: Characterization Using Conventional Flow Cytometry and Mass Cytometry

June 1, 2012

Author(s)

Lili Wang

To transform the linear fluorescence intensity scale obtained with fluorescent microspheres to an antibody bound per cell (ABC) scale, a biological cell reference material is needed. Optimally, this material should have a reproducible and tight ABC value

Point/Counterpoint: Put Simply - Standardization of Cardiac Troponin I is Complicated

November 29, 2011

Author(s)

David M. Bunk, Robert H. Christenson, Mauro Panteghini, Julan Barth, Alexei Katrukha, James Noble, Robert Porter, Heinz Schimmel, Jillian Tate, Lili Wang

None

Affinity maturation of human CD4 by yeast surface display and crystal structure of a CD4HLA-DR1 complex

October 7, 2011

Author(s)

Lili Wang, Xin Xiang Wang, Y Li, Min Mo, Yiyuan Yi, Qian Wang, W Gao, Roy A. Mariuzza

Helper T cell activation generally requires the co-receptor CD4, which binds MHC class II molecules. A remarkable feature of the CD4MHC class II interaction is its exceptionally low affinity, which ranges from KD ~200 μM to >2 mM. Investigating the

Development of Multicolor Flow Cytometry Standards: Assignment of Equivalent Reference Fluorophores (ERF) Unit

June 20, 2011

Author(s)

Lili Wang, Adolfas K. Gaigalas

A procedure is described for assigning values of the number of equivalent reference fluorophores (ERF) to microspheres labeled with a fluorophore designed to produce fluorescence in a given channel of a multicolor flow cytometer. There is a different

Negative Regulation of STAT3-mediated Cellular Respiration by SirT1

June 7, 2011

Author(s)

Lili Wang, Hua-Jun He, Michel Bernier, Rajib K. Paul, Alejandro Martin-Montalvo, Morten Scheibye-Knudsen, Shaoming Song, Sean M. Armour, Vilhelm A. Bohr, Yaping Zong, David A. Sinclair, Rafael de Cabo

In mammals, the transcriptional activity of signal transducer and activator of transcription 3 (STAT3) is regulated by the deacetylase SirT1. However, whether the newly described non-genomic actions of STAT3 toward mitochondrial oxidative phosphorylation

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