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Search Publications by: John Butler (Fed)

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Displaying 76 - 100 of 105

Multiplexed Assays for Evaluation of Y-SNP Markers in U.S. Populations

April 1, 2004
Author(s)
Peter Vallone, John Butler
Genetic markers located on the Y chromosome are of increasing importance in human identity testing. In an effort to evaluate the forensic utility of Y chromosome single nucleotide polymorphism (SNP) markers, we constructed several novel multiplex allele

Duplication of DYS19 Flanking Regions in Other Parts of the Y Chromosome

March 25, 2004
Author(s)
John M. Butler, R Schoske
During the testing of alternative primers for the Y chromosome short tandem repeat marker DYS19, a duplicated region of the Y chromosome was discovered. The duplicated sequence is contained within GenBank accession AC006335 and has a high degree of

Highly Multiplexed Assays for Measuring Polymorphisms on the Y-Chromosome

October 17, 2003
Author(s)
John M. Butler, R Schoske, Peter Vallone
A multiplex PCR assay capable of amplifying 20 Y chromosome short tandem repeat (STR) markers simultaneously has been developed. These markers include all of the Y STRs that make up the extended haplotype used in Europe (DYS19, DYS385, DYS389I/II, DYS390

A Novel Multiplex for Simultaneous Amplification of 20 Y Chromosome STR Markers

September 10, 2002
Author(s)
John M. Butler, R Schoske, Peter M. Vallone, Margaret C. Kline, A J. Redd, M F. Hammer
A multiplex PCR assay capable of simultaneously amplifying 20 Y-chromosome short tandem repeat (STR) markers has been developed to aid human identity testing and male population studies. These markers include all of the Y STRs that make up the extended

Polymerase Chain Reaction Amplification of DNA From Aged Blood Stains: Quantitative Evaluation of the Suitability for Purpose of Four Filter Papers as Archival Media

April 15, 2002
Author(s)
Margaret C. Kline, David L. Duewer, Janette W. Redman, John M. Butler, D A. Boyer
In collaboration with the Armed Forces Institute of Pathology's Department of Defense DNA Registry, the National Institute of Standards and Technology recently evaluated the performance of a short tandem repeat (STR) multiplex with dried whole blood stains

General Strategy for the Design of Multiplex PCR Assays Involving Y STR Markers

January 1, 2002
Author(s)
R Schoske, Peter Vallone, C M. Ruitberg, John Butler
The simultaneous amplification of multiple regions of a DNA template is routinely performed using the Polymerase Chain Reaction (PCR) in a process termed multiplex PCR. A useful strategy involving the design, testing and optimization of multiplex PCR

Development of Y STR Megaplex Assays

December 1, 2001
Author(s)
R Schoske, John Butler, Peter Vallone, Margaret C. Kline, M. Prinz, A J. Redd, M F. Hammer
Y Chromosome short tandem repeat markers have a number of applications in human identity testing including typing the perpetrator of sexual assault cases without differential extraction and tracing paternal lineages for missing person investigations. In

Quality Control of PCR Primers Used in Multiplex STR Amplifcation Reactions

June 1, 2001
Author(s)
John M. Butler, J E. Devaney, M A. Marino, Peter M. Vallone
Reliable amplification of short tandem repeat (STR) DNA markers with the polymerase chain reaction (PCR) is dependent on high quality PCR primers. The particular primer combinations and concentrations are especially important with multiplex amplification

Capillary Electrophoresis as a Tool for Optimization of Multiplex PCR Reactions

February 1, 2001
Author(s)
John M. Butler, C M. Ruitberg, Peter M. Vallone
Copying multiple regions of a DNA molecule is routinely performed today using the polymerase chain reaction (PCR) in a process commonly referred to as multiplex PCR. The development of a multiplex PCR reaction involves designing primer sets and examining