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Search Publications by: Carl G. Simon, Jr., Ph.D. (Fed)

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Displaying 51 - 75 of 265

Roles of Nanofiber Scaffold Structure and Chemistry in Directing Human Bone Marrow Stromal Cell Response

August 22, 2016
Author(s)
Sumona Sarkar, Bryan A. Baker, Desu Chen, Patrick S. Pine, Jennifer H. McDaniel, Marc L. Salit, Wolfgang Losert, Carl G. Simon Jr., Joy P. Dunkers
Nanofiber technology has emerged as a promising tool to recapitulate the native extracellular matrix structure; however the properties of nanofibers governing cell-material interactions are still largely undetermined. In this study we have systematically

Machine Learning Strategy to Determine Cell Shape Phenotypes Associated with Micro-Environmental Cues

June 25, 2016
Author(s)
Desu Chen, Sumona Sarkar, Julian Candia, Carl G. Simon Jr., Wolfgang Losert, Joy P. Dunkers, Stephen J. Florczyk, Subhadip Bodhak, Meghan Driscoll
Cell morphology has been identified as a potential indicator of stem cell response and function in biomaterial environments. However, determination of cell shape phenotype in biomaterials is complicated by heterogeneous cell populations, heterogeneous

Strategies for Achieving Measurement Assurance for Cell Therapy Products

June 1, 2016
Author(s)
Carl G. Simon Jr., Anne L. Plant, Sumona Sarkar, John T. Elliott, Sheng Lin-Gibson
Cell therapy products (CTPs) need quantitative, validated, and robust assays to support informed decision making during their development, manufacturing, and regulation. These products, the materials involved in their production and the methods for

3D Segmentation of Stem Cells from Thousands of Confocal Microscopy Images

March 1, 2016
Author(s)
Peter Bajcsy, Mylene H. Simon, Stephen J. Florczyk, Carl G. Simon Jr., Derek Juba, Mary C. Brady
We address the problem of estimating 3D segmentation performance when segmentation is applied to thousands of confocal microscopy images (z-stacks) of cells. With a very large number of z-stacks, manual inputs to validate each segmentation result are

Survey Statistics of Automated Segmentations Applied to Optical Imaging of Mammalian Cells

January 8, 2016
Author(s)
Peter Bajcsy, Antonio Cardone, Joe Chalfoun, Michael W. Halter, Derek Juba, Marcin Kociolek, Michael P. Majurski, Adele P. Peskin, Carl G. Simon Jr., Mylene H. Simon, Antoine Vandecreme, Anne L. Plant, Mary C. Brady
The goal of this survey paper is to overview cellular measurements using optical microscopy imaging followed by automated image segmentation. The cellular measurements of primary interest are taken from mammalian cells and their components. They are

ASTM Workshop on Standards & Measurements for Tissue Engineering Scaffolds

January 1, 2016
Author(s)
Carl G. Simon Jr., John Test, Michael Yaszemski, Reto Luginbuehl, Anthony Ratcliffe, Paul Tomlins
A “Workshop on Standards & Measurements for Tissue Engineering Scaffolds” was held on May 21, 2013 and was sponsored by the American Society of Testing and Materials International (ASTM International) in Indianapolis, IN. The purpose of the workshop was to

Validated Open Source Nanofiber Diameter Measurement Tool

April 30, 2015
Author(s)
Nathan A. Hotaling, Kapil Bharti, Haydn Kriel, Carl G. Simon Jr.
Despite the growing use of nanofiber scaffolds for tissue engineering applications, there is not a validated, readily available (commercial or open source) solution for rapid, automated analysis of nanofiber diameter from scanning electron microscope (SEM)

Ontology Analysis if Global Gene Expression Differences of Human Bone Marrow Stromal Cells Cultured on 3D Scaffolds or 2D Films

July 31, 2014
Author(s)
Bryan A. Baker, Patrick S. Pine, Kaushik Chatterjee, Girish Kumar, Jennifer H. McDaniel, Marc L. Salit, Carl G. Simon Jr.
Differences in gene expression of human bone marrow stromal cells (hBMSCs) during culture in three-dimensional (3D) nanofiber scaffolds or on two-dimensional (2D) films were investigated via pathway analysis of microarray mRNA expression profiles. Previous

Combinatorial Cassette for Screening of Osteogenesis by Transplanted Human Bone Marrow Stromal Cells

April 19, 2014
Author(s)
Subhadip Bodhak, Sheng Lin-Gibson, Carl G. Simon Jr., Luis Fernandez de Castro Diaz, Pamela G. Robey, Sergei A. Kuznetsov, Tina Kilts, Marian F. Young
The use of in vivo transplantation assays under defined experimental conditions has become a valuable standard for evaluating osteogenic potential of different stem cell populations and is the subject of ongoing pre-clinical reconstructive therapy research