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Search Publications by: Joseph Curtis (Fed)

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Displaying 1 - 25 of 90

Anisotropic coarse-grain Monte Carlo simulations of lysozyme, lactoferrin, and NISTmAb by precomputing atomistic models

September 5, 2024
Author(s)
Harold Hatch, Christina Bergonzo, Marco Blanco, Guangcui Yuan, Sergei Grudinin, Mikael Lund, Joseph E. Curtis, Alexander Grishaev, Yun Liu, Vincent K. Shen
We develop a multiscale coarse-grain model of the NIST Monoclonal Antibody Reference Material 8671 (NISTmAb) to enable systematic computational investigations of high-concentration physical instabilities such as phase separation, clustering, and

Morphological Characterization of Self-Amplifying mRNA Lipid Nanoparticles

January 16, 2024
Author(s)
Jacob Thelen, Wellington Leite, Volker Urban, Hugh O'Neill, Alexander Grieshaev, Joseph E. Curtis, Susan N. Krueger, Maria M. Castellanos
The mRNA technology has emerged as a rapid modality to develop vaccines during pandemic situations with the potential to protect against endemic diseases. The success of mRNA in producing an antigen is dependent on the ability to deliver mRNA to the cells

Role of Domain-Domain Interactions on the Self-Association and Physical Stability of Monoclonal Antibodies: Effect of pH and Salt

September 26, 2023
Author(s)
Amy Xu, Marco Blanco, Maria Castellanos, Curtis Meuse, Kevin Mattison, Ioannis Karageorgos, Harold Hatch, Vincent Shen, Joseph E. Curtis
Monoclonal antibodies (mAbs) make up a major class of biotherapeutics with a wide range of clinical applications. Their physical stability can be affected by various environmental factors. For instance, an acidic pH can be encountered during different

Styrene-Maleic Acid Copolymer Nanodiscs to Determine the Shape of Membrane Proteins

January 28, 2022
Author(s)
Cheol Jeong, Ryan Franklin, Karen J. Edler, Kenno Vanommeslaeghe, Joseph E. Curtis
Lipid nanodiscs can be used to solubilize functional membrane proteins (MPs) in nativelike environments. Thus, they are promising reagents that have been proven useful to characterize MPs. Both protein and non-protein molecular belts have shown promise to

Intermediate Scattering Functions of a Rigid Body Monoclonal Antibody Protein in Solution Studied by Dissipative Particle Dynamic Simulation

April 8, 2021
Author(s)
Yanqin Zhai, Nicos Martys, William L. George, Joseph E. Curtis, Jannatun Nayem, Y. Z, Yun Liu
In the past decade, there is increased research interest in studying internal motions of flexible proteins in solution using Neutron Spin Echo (NSE) as NSE can simultaneously probe the dynamics at the length and time scale comparable to protein domain

Structural Characterization and Modeling of a Respiratory Syncytial Virus Fusion Glycoprotein Nanoparticle Vaccine in Solution

January 4, 2021
Author(s)
Susan T. Krueger, Joseph E. Curtis, Daniel R. Scott, Alexander Grishaev, Greg Glenn, Gale Smith, Larry Ellingsworth, Oleg Borisov, Ernest Maynard
Globally, respiratory syncytial virus (RSV)is a major cause of sever lower respiratory tract infection in young children, older adults, and immune compromised populations, for which there are no licensed vaccines. The RSV fusion (F)/polysorbate 80 (PS80)

Characterization of an Extensive Interface on Vitronectin for Binding to Plasminogen Activator Inhibitor-1: Adoption of Structure in an Intrinsically Disordered Region

December 24, 2019
Author(s)
Letitia O. Puster, Christopher B. Stanley, Vladmir N. Uversky, Joseph E. Curtis, Susan T. Krueger, Yuzhuo Chu, Cynthia B. Peterson
Small-angle neutron scattering (SANS) measurements were used to characterize vitronectin, a protein found in human plasma and tissues that functions in regulating cell adhesion and migration, as well as proteolytic cascades that affect blood coagulation

ROTDIF-web and ALTENS: GenApp-based Science Gateways for Biomolecular Nuclear Magnetic Resonance (NMR) Data Analysis and Structure Modeling

September 23, 2019
Author(s)
Yuexi Chen, Cheol Jeong, Alexey Savelyev, Susan T. Krueger, Joseph E. Curtis, Emre H. Brookes, David Fushman
Proteins and nucleic acids participate in essentially every biochemical process in living organisms, and the elucidation of their structure and motions is essential for our understanding how these molecular machines perform their function. Nuclear Magnetic

BEES: Bayesian Ensemble Estimation from SAS

August 6, 2019
Author(s)
Samuel Bowerman, Joseph E. Curtis, Joseph Clayton, Emre H. Brookes, Jeff Wereszczynski
Many biomolecular complexes exhibit a flexible ensemble of conformations in solution in order to conduct their biological function. Small angle scattering (SAS) measurements are a popular method for characterizing these flexible molecules due to their