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Search Publications by: Young Jong Lee (Fed)

Displaying 1 - 25 of 79

Determining 3D molecular orientation from polarization-IR spectra: tutorial

January 3, 2025

Author(s)

Young Jong Lee

Despite the ubiquity of molecular alignment in natural and synthesized materials, accurate mapping of three-dimensional (3D) molecular orientations with sufficient spatial resolving power has remained challenging. Conventional analysis approaches of

Benchtop IR Imaging of Live Cells: Monitoring the Total Mass of Biomolecules in Single Cells

September 4, 2024

Author(s)

Yow-Ren Chang, Seongmin Kim, Young Jong Lee

Absolute quantity imaging of biomolecules on a single cell level is critical for measurement assurance in biosciences and bioindustries. While infrared (IR) transmission microscopy is a powerful label-free imaging modality capable of chemical

Single-Detector Double-Beam Modulation for High-Sensitivity Infrared Spectroscopy

October 25, 2023

Author(s)

Seongmin Kim, Yow-Ren Chang, Young Jong Lee

Balanced detection based on double beams is widely used to reduce common-mode noises, such as laser intensity fluctuation and irregular wavelength scanning, in absorption spectroscopy. However, employing an additional detector can increase the total system

3D Orientation Imaging of Polymer Chains with Polarization-Controlled Coherent Raman Microscopy

December 7, 2022

Author(s)

Shuyu Xu, Young Jong Lee

Despite the ubiquity of three-dimensional (3D) anisotropic materials, their 3D molecular alignment cannot be measured using conventional two-dimensional (2D) polarization imaging. Here, we present images of the 3D angles of molecular orientations with

Effects of Salts and Surface Charge on the Biophysical Stability of a low pI Monoclonal Antibody

November 15, 2022

Author(s)

Kelvin B. Rembert, Young Jong Lee, Jifeng Zhang

The impact of five representative Hofmeister salts (NaCl, KCl, MgCl2, Na2SO4, and NaSCN) on the thermal stability and aggregation kinetics of a slightly acidic monoclonal antibody (mAb) were investigated under different pH conditions. The thermal stability

Coherent anti-Stokes Raman scattering (CARS) microscopy for polymers

April 1, 2022

Author(s)

Shuyu Xu, Charles Camp, Young Jong Lee

Coherent anti-Stokes Raman scattering (CARS) microscopy is a label-free chemical imaging modality capable of interrogating local molecular composition, concentration, and even orientation. In comparison to traditional Raman spectroscopy/imaging, CARS

Three-Dimensional Molecular Orientation Imaging of a Semicrystalline Polymer Film under Shear Deformation

March 31, 2022

Author(s)

Young Jong Lee, Shuyu Xu, Chad R. Snyder, Jeremy Rowlette

The 3D molecular orientations of polymer chains are imaged and compared for a quiescent spherulite region and a shear deformed region of a semicrystalline poly(e-caprolactone) (PCL) film. The images of 3D orientation are acquired by a newly developed

Imaging 3D molecular orientation by orthogonal-pair polarization IR microscopy

February 28, 2022

Author(s)

Shuyu Xu, Chad R. Snyder, Jeremy Rowlette, Young Jong Lee

Anisotropic molecular alignment occurs ubiquitously and often heterogeneously in three dimensions (3D). However, conventional imaging approaches based on polarization can map only molecular orientation projected onto the 2D polarization plane. Here, an

Real-time and high-throughput Raman signal extraction and processing in CARS hyperspectral imaging

June 9, 2021

Author(s)

Charles Camp, John S. Bender, Young Lee

We present a new collection of processing techniques, collectively "factorized Kramers-Kroenig and error correction" (fKK-EC), for (a) Raman signal extraction, (b) denoising, and (c) phase- and scale- error correction in coherent anti-Stokes Raman

Compensation of Strong Water Absorption in Infrared Spectroscopy Reveals the Secondary Structure of Proteins in Dilute Solutions

January 12, 2021

Author(s)

Bonghwan Chon, Shuyu Xu, Young Jong Lee

Infrared (IR) absorption spectroscopy is a powerful tool that can quantify complex biomolecules and their structural conformations. However, conventional approaches to protein analysis in aqueous solutions have been significantly challenged because the

Theory of Birefringence Correction for Polarization-Controlled CARS

March 16, 2020

Author(s)

Young J. Lee

Polarization-controlled coherent Raman spectroscopy is used as a high-throughput method to characterize the anisotropic nature of a molecular system, such as the molecular orientation distribution. However, optical birefringence originating from the

Position- and Polarization-Specific Waveguiding of Multi-Emissions in Single ZnO Nanorods

June 19, 2019

Author(s)

Bonghwan Chon, Johnson Truong, Matthew Hansen, Jong-in Hahm, Young Jong Lee

We examine multiphoton-produced optical signals waveguided through single ZnO nanorods (NRs) using a newly developed, scanning offset-emission hyper- spectral microscopy (SOHM) technique. Specifically, we concurrently analyze waveguiding behaviors of sum

Concurrent polarization IR analysis to determine the 3D angles and the order parameter for molecular orientation imaging

September 5, 2018

Author(s)

Young J. Lee

A non-tomographic approach based on polarization-dependent infrared (IR) spectroscopy is proposed for a simultaneous measurement of the 3D angles and the orientational order parameter of a molecular orientation distribution. Unlike conventional

Stoichiometric analysis of hydrogen bonding interactions in a mixture of cross-linking resin monomers

June 27, 2018

Author(s)

Seungwan Ryu, Xiaohui Liu, Ying Jin, Jirun Sun, Young Jong Lee

We investigate multiple hydrogen bonding interactions in a binary mixture of cross-linking resin monomers: urethane dimethacrylate (UDMA) and triethylene glycol-divinylbenzyl ether (TEG-DVBE). We analyze infrared (IR) absorption spectra observed at various