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Search Publications by: David P. Hoogerheide (Fed)

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Displaying 1 - 25 of 57

Maximally efficient exchange in thin flow cells using density gradients

October 25, 2024
Author(s)
Megan Mitchell, Charles Majkrzak, David Hoogerheide
Flow cells are ubiquitous in laboratories and automated instrumentation and are crucial for ease of sample preparation, analyte addition, and buffer exchange. Often, the assumption that the fluids have exchanged completely in a flow cell is crucial to data

AutoRefl: Active Learning in Neutron Reflectometry for Fast Data Acquisition

August 1, 2024
Author(s)
David Hoogerheide, Frank N. Heinrich
Neutron reflectometry (NR) is a powerful technique for interrogating the structure of thin films at interfaces. Because NR measurements are slow and instrument availability is limited, measurement efficiency is paramount. One approach to improving

Restricting a-Synuclein Transport into Mitochondria by Inhibition of a-Synuclein-VDAC Complexation as a Potential Therapeutic Target for Parkinson's Disease Treatment

October 1, 2022
Author(s)
Megha Rajendran, Maria Queralt-Martin, Philip A. Gurnev, William M. Rosencrans, Amandine Rovini, Daniel Jacobs, Kaitlin Abrantes, David Hoogerheide, Sergey M. Bezrukov, Tatiana Rostovtseva
Involvement of alpha-synuclein (αSyn) in Parkinson's disease (PD) is complicated and difficult to trace on cellular and molecular levels. Recently we established that αSyn can regulate mitochondrial function by voltage-activated complexation with the

The Single Residue K12 Governs the Exceptional Voltage Sensitivity of Mitochondrial Voltage-Dependent Anion Channel Gating

August 17, 2022
Author(s)
Van Ngo, Maria Queralt-Martin, Farha Khan, Lucie Bergdoll, Jeff Abramson, Sergey M. Bezrukov, Tatiana Rostovtseva, David Hoogerheide, Sergei Noskov
The voltage-dependent anion channel (VDAC) is a β-barrel channel of the mitochondrial outer membrane (MOM) that passively transports ions, metabolites, polypeptides, and single-stranded DNA. VDAC responds to a transmembrane potential by transitioning to a

Low-Background Neutron Reflectometry from Solid/Liquid Interfaces

February 1, 2022
Author(s)
David Hoogerheide, Joseph Dura, Brian B. Maranville, Charles F. Majkrzak
Liquid cells are an increasingly common sample environment for neutron reflectometry experiments and are critical for measuring the properties of materials at solid/liquid interfaces. Background scattering determines the maximum useful scattering vector

Highly Basic Clusters in the Herpes Simplex Virus 1 Nuclear Egress Complex Drive Membrane Budding by Inducing Lipid Ordering

August 24, 2021
Author(s)
Michael K. Thorsen, Alex Lai, Michelle W. Lee, David Hoogerheide, Gerard C. Wong, Jack H. Freed, Ekaterina Heldwein
During replication of herpesviruses, capsids escape from the nucleus into the cytoplasm by budding at the inner nuclear membrane. This process is mediated by the viral nuclear egress complex (NEC), but how the NEC interacts with lipids or generates

VDAC Gating Thermodynamics, but Not Gating Kinetics, Are Virtually Temperature Independent

December 15, 2020
Author(s)
Maria Queralt-Martin, David Hoogerheide, Sergei Y. Noskov, Alexander M. Berezhkovskii, Tatiana Rostovtseva, Sergey Bezrukov
The voltage-dependent anion channel, VDAC, is the most abundant protein in the mitochondrial outer membrane and an archetypical β-barrel channel. Here, we study the effects of temperature on VDAC channels reconstituted in planar lipid membranes at the