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PUBLICATIONS

Single-cell measurement of plasmid copy number and promoter activity

Published

Author(s)

Bin Shao, Jayan Rammohan, Daniel Anderson, Nina Alperovich, David J. Ross, Christopher Voigt

Abstract

We have developed a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.
Citation
Nature Communications
Pub Type
Journals

Download Paper

https://doi.org/10.1038/s41467-021-21734-y
Local Download

Keywords

Synthetic Biology, Biophysics, Design Automation, Metrology, Abstraction, Standards
Engineering / synthetic biology, Cell measurements, Bioscience and Biological physics

Citation

Shao, B. , Rammohan, J. , Anderson, D. , Alperovich, N. , Ross, D. and Voigt, C. (2021), Single-cell measurement of plasmid copy number and promoter activity, Nature Communications, [online], https://doi.org/10.1038/s41467-021-21734-y, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=930181 (Accessed February 5, 2025)

Issues

If you have any questions about this publication or are having problems accessing it, please contact reflib@nist.gov.

Created March 5, 2021, Updated October 12, 2021