Biological physics

News and Updates

Spotlight: Game-Changing Microscopy Technique for Identifying Cancerous Cells

September 3, 2024

Breast-conserving surgery, or lumpectomy, is a treatment for early-stage breast cancer. It’s important for surgeons to remove enough tissue around the tumor to

NIST Researchers Develop a Breakthrough COVID-19 Detection Technology

October 2, 2023

“Only connect” is the most famous line in the British novelist E. M. Forster’s writings. Forster was urging the readers of his book Howard’s End to make

white matter mimic in the bottom half of the artificial cranium

Lessons from an Experimental Brain Phantom

July 12, 2023

Researchers at the National Institute of Standards and Technology (NIST) and colleagues have developed a novel, anatomically accurate reference model of the

Projects and Programs

viral vector illustration with RNA seen within the capsid

Gene Delivery Particle Analysis with High-Throughput Light Scattering Microscopy

Ongoing

This project aims to develop light-scattering microscopy methods for rapid, multi-attribute characterization of gene therapy particles at the single particle level. Emerging gene therapy treatments use gene delivery particles, such as viral vectors and non-viral carriers, to cure intractable

Single-molecule biosensors

Ongoing

We are developing new tools to investigate the energy landscape of single-molecule sensors. Our goal is to apply a wide-ranging array of technologies to probe the critical physicochemical properties of nanopore biosensors. These properties include the free energy of confinement for polymers as they

Calibration Services: National Air-Kerma Standard for Electronic Brachytherapy

Ongoing

The increased use of limited-lifetime electronic brachytherapy sources which are miniature, low-energy x-ray tubes for clinical applications resulted in the need of a measurement of air-kerma dose from NIST. The type of electronic brachytherapy sources, Axxent S700, used at NIST operates at tube

Nano-biophotonics for molecular imaging

Ongoing

Nano-biophotonics consists of four broad areas: molecular bioimaging; nano-biosensors; multiplexed bioassays ; and nanotechnology-based medical practices for diagnosis and therapy. Success in these areas is challenged by the underlying complexity of biological systems. Major levels of complexity and

Publications

Quantifying a light-induced energetic change in bacteriorhodopsin by force spectroscopy

February 7, 2024

Author(s)

David Jacobson, Thomas T. Perkins

Influence of fluorescence lifetime selections and conformational flexibiiliy on brightness of FusionRed variants

February 5, 2024

Author(s)

Srijit Mukherjee, Nancy Douglas, Ralph Jimenez

Excited State Vibrational Dynamics Reveals a Photocycle That Enhances the Photostability of the TagRFP-T Fluorescent Protein

January 28, 2024

Author(s)

Atsushi Yabushita, Ying Kuan Ko, Takayoshi Kobayashi, Izumi Iwakura, Ralph Jimenez

Narrowing Signal Distribution by Adamantane Derivatization for Amino Acid Identification Using α-Hemolysin Nanopore

January 24, 2024

Author(s)

Xiaojun Wei, Dumei Ma, Junlin Ou, Ge Song, Jiawei Guo, Joseph Robertson, Yi Wang, Qian Wang, Chang Liu

Nanopores have demonstrated remarkable sensitivity as biosensors, enabling the sequencing of DNA and RNA at the single-molecule level. The rapid progress in

Software

NIST's Integrated Colony Enumerator (NICE)

January 4, 2021

Enumeration of bacterial colonies on an agar plate is simple in concept, but automated colony counting is difficult due to variations in colony color, size

MOSAIC

MOSAIC is a modular single-molecule analysis toolbox to decode multi-state single-molecule and nanopore time-series data. Read the documentation for additional