Gallagher, D.
, Godoy-Ruiz, R.
, Krejcirikova, A.
and Tugarinov, V.
(2011),
Solution NMR Evidence for Symmetry in Functionally or Crys-tallographically Asymmetric Homodimers, Journal of the American Chemical Society, [online], https://doi.org/10.1021/ja206967d
(Accessed December 26, 2024)
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Solution NMR Evidence for Symmetry in Functionally or Crys-tallographically Asymmetric Homodimers
Published
Author(s)
, Raquel Godoy-Ruiz, Anna Krejcirikova, Vitali Tugarinov
Abstract
A recurrent theme of many structural studies of homo-oligomeric protein systems is concerned with verification that the conformation observed in a crystal repre¬sents the functionally relevant structure. An asymmetric con-formation adopted by two chemically identical subunits in homo-oligomers can represent an intrinsic property of a protein or be an artifact induced by crystal packing forces. Solution NMR studies can distinguish between these two possibilities. Using methyl-based NMR spectroscopy we provide evidence for symmetry in the absence of ligands in several homodimeric proteins that are either asymmetric functionally and/or adopt different conformations of the two subunits in available x-ray structures.Citation
Journal of the American Chemical Society
Volume
133
Issue
49
Pub Type
Journals
Download Paper
Keywords
NMR, CRP, Symmetry, Crystallography
Citation
Issues
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Created October 18, 2011, Updated March 14, 2023