Bioimaging

Orthogonal Measurements

Ongoing

Definitions: Orthogonal measurements: Measurements that use different physical principles to measure the same property of the same sample with the goal of minimizing method-specific biases and interferences. Complementary measurements: Measurements that corroborate each other to support the same

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

Biofilm Models to Evaluate Structure-Function Relationships

Ongoing

Biofilms are complex 3D microbial structures that exist throughout nature including on our skin and gut mucosa, in waterways, and in soil. They are typically composed of multiple microbial species surrounded by proteins, polysaccharides, and extracellular DNA woven together into a hydrated matrix

This figure shows a microscopic image of a field of cells, some of which are pseudocolored red to indicate that these cells remain pluripotent.

Stem Cell Metrology: WSDOM

Ongoing

Stem cells are critical starting materials for advanced therapies and diagnostic devices. The most commonly used are induced pluripotent stem cells (iPSCs) which can be created with any individual’s skin cells, and then can be coaxed to become cells for any tissue of the body. Therapies and

News and Updates

A hand in a purple glove turns a metal dial on a plate holding tiny vials of purple liquid.

NIST Develops New Nondestructive Method for Assessing Bioengineered Artificial Tissues

Stem Cells and AI: Better Together

Images of the same basic cell type grown on two different bioscaffolds

Bioimaging

The Research

Orthogonal Measurements

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Biofilm Models to Evaluate Structure-Function Relationships

Stem Cell Metrology: WSDOM

News and Updates

NIST Develops New Nondestructive Method for Assessing Bioengineered Artificial Tissues

Stem Cells and AI: Better Together

Shape-Sifting: NIST Categorizes Bio Scaffolds by Characteristic Cell Shapes