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Bioscience

Bioimaging

Projects/Programs

Displaying 1 - 12 of 12

Bioimaging

Completed
Imaging and image analysis are used ubiquitously in biological and biomedical research. One of the great advantages of imaging is its intuitive information content; much can be learned from just a glance at the data. On the other hand, these data generally contain a very large amount of untapped

Cell-based Assay for Mechanistically-based Prediction of Cytoxicity

Completed
Ricin is a type 2 ribosome inactivating protein (RIP) belonging to the A-B family of toxins and is of particular interest because of its potential use as a biological weapon and the dearth of rapid and sensitive assays that could assist detection and decontamination. The ricin toxin inhibits protein
microfluidic chip

Evaluating Measurement Quality of Microfluidic Cell-based Assays

Completed
Impact and Customers Our work establishes metrics for measurement quality and reproducibility for cell-based assays within microfluidic devices. Many novel microfluidic devices are being created that permit new measurements or enable new biological functions to be tested; however, characterizations
Fluorescence benchmarking

Fluorescence Microscopy Benchmarking: Overview

Completed
A challenge in using microscopy is how to compare and validate data from different instruments, and how to determine that the microscope performance today is the same as yesterday. NIST is developing a practical, automated approach to implementing performance benchmarking methods that will

Quantitative, Cell-based Immunofluorescence Assays

Completed
Analysis of signaling events within individual cells with microscopy imaging has several advantages over non-imaging techniques. The principal advantage of imaging cytometry over plate reader assays and even other single cell techniques such as flow cytometry is the potential for collection of

Quantum Bioimaging

Ongoing
Our efforts in BBD are focused on using the quantum nature of light to facilitate enhanced and novel measurement technologies for biological samples. For example, so-called bright squeezed laser sources enable imaging and sensing with less noise than is classically possible. Additionally, entangled
This figure shows a microscopic image of a field of cells, some of which are pseudocolored red to indicate that these cells remain pluripotent.

Stem Cell Metrology: WSDOM

Ongoing
Stem cells are critical starting materials for advanced therapies and diagnostic devices. The most commonly used are induced pluripotent stem cells (iPSCs) which can be created with any individual’s skin cells, and then can be coaxed to become cells for any tissue of the body. Therapies and