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Material Measurement Laboratory / Biomolecular Measurement Division

Applied Genetics Group

Projects/Programs

Displaying 1 - 14 of 14
Bioman lifecycle

Biomanufacturing Initiative

Ongoing
The objective of the NIST biomanufacturing program is to support US biopharmaceutical industry delivery of high quality and low cost protein drugs around the world. The mission is fulfilled through the development of standards, measurement science, and state of the art tools that support advances in

Certification of a Standard Reference Material for Cytomegalovirus (CMV)

Ongoing
The goal of this project is to provide a SI-traceable reference material for use by calibrant and reagent manufacturers in production of their own calibrants and standards. Clinical laboratories with in-house methods would be able to use the reference material for production of their own quality

Development of Rapid Multiplex PCR Techniques

Ongoing
With the continuing development of miniaturization technologies such as microfluidic and micro-capillary devices there is a desire to reduce the overall time required to type DNA samples. Such miniature devices could be used for initial screening at a crime scene, at a border, or at airports. There

Digital PCR

Ongoing
With appropriately validated assays, digital polymerase chain reaction (dPCR) determines the number of DNA targets (copies) per reaction partition, without the need for a standard curve. Reaction partitions can be in the form of fixed chambers in a microfluidic device, referred to as chamber digital

DNA Biometrics

Ongoing
Our focus is on developing methods to decrease the time required to perform a DNA test and assisting with the evaluation of new prototype rapid DNA typing instrumentation. The use of rapid DNA testing as a biometric mode would impact the areas of immigration, airport and border security (Dept of

DNA Mixture Interpretation

Ongoing
Mixtures of DNA from more than one source are commonly seen in forensic evidentiary samples and are challenging to interpret. Over the past decade, our group at NIST has conducted several interlaboratory studies involving mixture interpretation with short tandem repeat (STR) markers in order to

DNA Profiling Standard Reference Materials

Ongoing
Intended impact This work was designed to provide materials that forensic laboratories being assessed under the FBI DNA Quality Assurance Standard can comply with as stated, "The laboratory shall check its DNA procedures annually or whenever substantial changes are made to the protocol(s) against an
CE and sequencing results for STR D3S1358 an allele 15 example

Forensic Applications of Next Generation Sequencing

Ongoing
Forensic DNA profiles have historically been generated by Capillary Electrophoresis (CE), which results in length-based designations reported as the number of repeats at STR (short tandem repeat) markers. For example, the marker D3S1358 contains repeats of the four nucleotides “TCTA” or “TCTG”. When
Unit of RGTM 10263 -- H5N1 Avian flu synthetic RNA fragments

H5 Influenza Positive Controls

Ongoing
In response to the current H5N1 avian influenza outbreak, NIST has developed a research grade test material (RGTM) for PCR-based assays. This material contains 3 vials with RNA fragments for HA (Part A), NA (Part B), and MP (Part C). A NIST Reference Material is also under development. The guidance
Cryogenic electron microscopy (cryo-EM) image of an LNP/RNA formulation

LNP-RNA Research Grade Test Material

Ongoing
The currently planned LNP/RNA material (designated RGTM 10240) will include a fragment of the SARS-CoV-2 genome that is approximately 4 kb in length. This fragment was previously included (in unencapsulated form) as “Fragment 1” of NIST RGTM 10169. The RNA fragment is not designed for cell-based

NIST Monoclonal Antibody Reference Material 8671

Ongoing
The NISTmAb material is a recombinant humanized IgG1κ expressed in murine suspension culture. It is an »150 kDa homodimer of two identical light chains and two identical heavy chains linked through both inter- and intra-chain disulfide bonds. The protein has low abundance post-translational
Epidemiologist in protective suit, mask and glasses works with patient swabs to detect specific region of 2019-nCoV virus causing Covid-19

SARS-CoV-2 Research Grade Test Material

Completed
In response to the COVID-19 pandemic, the National Institute of Standards and Technology released a synthetic RNA material for SARS-CoV-2 in June 2020. The goal was to rapidly produce a material to support molecular diagnostic testing applications. This material, referred to as Research Grade Test
BK_JC_plasmids

BK and JC Virus DNA Standard Reference Materials

Ongoing
Whole BKV or JCV virus genomes have been inserted into a cloning vector, propagated in Escherichia coli, extracted, linearized, and certified for number of copies of the genome per volume (e.g. copies per microliter). The DNA was sequenced and provided as information value in the certificate of