Bioimaging

Orthogonal Measurements

Ongoing

Definitions: Orthogonal measurements: Measurements that use different physical principles to measure the same property of the same sample with the goal of minimizing method-specific biases and interferences. Complementary measurements: Measurements that corroborate each other to support the same

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

Measuring Cell Viability in Scaffolds

Ongoing

Project Goals Primary Goal: Advance measurements of cell viability in manufactured tissues Establish working group of industry stakeholders to guide work Develop model scaffold-cell-assay system for measuring cell viability in scaffolds Validate measurement system by orthogonal test methods Assess

Standards for Immuno-histochemical Imaging: A Biomarker Protein Expression Chip

Completed

Intended Impact The lack of quantitative imaging methods and reagents impedes the validation of image-based biomarkers. Improved methods for biomarker quantification and an established traceable value assignment would be useful to customers in a wide range of areas that include clinical diagnostics

News and Updates

A hand in a purple glove turns a metal dial on a plate holding tiny vials of purple liquid.

Bioimaging

The Research

Orthogonal Measurements

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Measuring Cell Viability in Scaffolds

Standards for Immuno-histochemical Imaging: A Biomarker Protein Expression Chip

News and Updates

NIST Develops New Nondestructive Method for Assessing Bioengineered Artificial Tissues

A Recap of TMAP

NIST Scientists and Engineers Pitch Innovations to Venture Capitalists