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Jagat Budhathoki, Ph.D. (Fed)

Physical Scientist

Pronouns

Cell Systems Science Group

jagat.budhathoki [at] nist.gov

(301) 975-3092

Staff Employment

Postdoctoral Fellow, National Institute of Health, 2020 – 2024

Post-doctoral Research Associate, Department of Molecular Biology and Genetics, Cornell University, 2016 – 2020

Staff Education

Ph.D. Biophysics, Kent State University, Kent, OH, 2016

M.Sc., Physics, Tribhuvan University, Nepal, 2008

B.Sc., Physics, Patan Multiple Campus, Nepal, 2006

Areas of Expertise

Bioimaging

Gene delivery systems

Biological physics

Optical physics and communications

Spectroscopy

Jagat works on an IMS project to characterize gene delivery particles, with a focus on measuring size, number concentration, affinity, and composition to support advancements in gene therapy manufacturing.

During his postdoctoral research at the National Institutes of Health (NIH), he developed single-molecule assays to study DNA condensation by ParB proteins and bulk assays to investigate cell division-regulating systems like the Min System. At Cornell University, Jagat explored CRISPR adaptation and DNA repair mechanisms.

Prior to that, Jagat’s graduate research focused on single-molecule fluorescence resonance energy transfer (smFRET), studying the folding and unfolding of G-quadruplex structures by RecQ helicases. During this time, he developed expertise in building TIRF imaging systems, surface passivation for noise suppression, protein expression and purification, and advanced image and data analysis.