Bioimaging

Orthogonal Measurements

Ongoing

Definitions: Orthogonal measurements: Measurements that use different physical principles to measure the same property of the same sample with the goal of minimizing method-specific biases and interferences. Complementary measurements: Measurements that corroborate each other to support the same

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

Advanced Spectral Analysis: Two-Dimensional Correlation Spectroscopy (2DCOS)

Ongoing

When a series of spectra are acquired from a sample as a function of temperature, pressure, electric field strength, concentration, or any experimental parameters, conventional spectral analysis is performed by monitoring the intensity change at a specific frequency. However, such a single frequency

MML researchers develop reference materials and methods for quantitative phase microscopy with off-the-shelf products

Measurement Assurance for Quantitative Cell Imaging by Optical Microscopy

Ongoing

Light microscopy is unparalleled as a quantitative technique for studying cell biology, but assuring that the results of measurements and analyses are accurate, repeatable and sharable is challenging. Here we provide information on reference materials, protocols and approaches for evaluating the

News and Updates

A hand in a purple glove turns a metal dial on a plate holding tiny vials of purple liquid.

NIST Develops New Nondestructive Method for Assessing Bioengineered Artificial Tissues

Stem Cells and AI: Better Together

Images of the same basic cell type grown on two different bioscaffolds

Bioimaging

The Research

Orthogonal Measurements

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Advanced Spectral Analysis: Two-Dimensional Correlation Spectroscopy (2DCOS)

Measurement Assurance for Quantitative Cell Imaging by Optical Microscopy

News and Updates

NIST Develops New Nondestructive Method for Assessing Bioengineered Artificial Tissues

Stem Cells and AI: Better Together

Shape-Sifting: NIST Categorizes Bio Scaffolds by Characteristic Cell Shapes