Bioimaging

Quantitative, Cell-based Immunofluorescence Assays

Completed

Analysis of signaling events within individual cells with microscopy imaging has several advantages over non-imaging techniques. The principal advantage of imaging cytometry over plate reader assays and even other single cell techniques such as flow cytometry is the potential for collection of

Comparing the Biological Responses of Photoluminescent Silicon Nanoparticles with Silicon Micron-sized Particles

Completed

Intended Impact Technology is now available to produce nanoparticles of uniform size and shape which may allow their use in a wide range of applications from drug delivery to water purification. The high surface area to volume ratio of nanoparticles makes them particularly good catalysts as well as

Standards for Immuno-histochemical Imaging: A Biomarker Protein Expression Chip

Completed

Intended Impact The lack of quantitative imaging methods and reagents impedes the validation of image-based biomarkers. Improved methods for biomarker quantification and an established traceable value assignment would be useful to customers in a wide range of areas that include clinical diagnostics

TOP: Image of fluorescent reference material on microscope. BOTTOM: Charting of microscope control parameters.

Performance Benchmarking and Intensity Calibration of a Widefield Fluorescence Microscope

Ongoing

Widefield fluorescence microscopy is a highly used tool for visually assessing biological samples and for quantifying cell responses. Despite its widespread use in high content analysis and other imaging applications, few published methods exist for evaluating and benchmarking the analytical

Bioimaging

The Research