Bioimaging

Orthogonal Measurements

Ongoing

Definitions: Orthogonal measurements: Measurements that use different physical principles to measure the same property of the same sample with the goal of minimizing method-specific biases and interferences. Complementary measurements: Measurements that corroborate each other to support the same

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Ongoing

The current gold standard for cell viability measurements is the trypan blue assay. Trypan blue dye enters dead cells that have a ruptured membrane. As a result, the dead cells are stained blue. Live cells with an intact membrane, on the other hand, do not absorb the dye molecules and remain

Bioprocessing Laboratory

Ongoing

Current Capabilities Computer controlled liquid nitrogen biostorage system Two culture areas including 4 monitored CO2 incubators 2 Six-foot BSLII biosafety cabinets Measurements- cell count and concentration, STR typing, flow cytometry, cell volume size distribution, conventional transmission and

Standards for Immuno-histochemical Imaging: A Biomarker Protein Expression Chip

Completed

Intended Impact The lack of quantitative imaging methods and reagents impedes the validation of image-based biomarkers. Improved methods for biomarker quantification and an established traceable value assignment would be useful to customers in a wide range of areas that include clinical diagnostics

News and Updates

A hand in a purple glove turns a metal dial on a plate holding tiny vials of purple liquid.

NIST Develops New Nondestructive Method for Assessing Bioengineered Artificial Tissues

Stem Cells and AI: Better Together

Images of the same basic cell type grown on two different bioscaffolds

Bioimaging

The Research

Orthogonal Measurements

Quantitative, Traceable Determination of Cell Viability Using Absorbance Microscopy

Bioprocessing Laboratory

Standards for Immuno-histochemical Imaging: A Biomarker Protein Expression Chip

News and Updates

NIST Develops New Nondestructive Method for Assessing Bioengineered Artificial Tissues

Stem Cells and AI: Better Together

Shape-Sifting: NIST Categorizes Bio Scaffolds by Characteristic Cell Shapes