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Displaying 1 - 25 of 80

Glucocorticoids Alter Bone Microvascular Barrier via MAPK/Connexin43 Mechanisms

January 20, 2025
Author(s)
Eun Jin Lee, Peter Lialios, Micaila Curtis, James Williams IV, Yoontae Kim, Paul Salipante, Steven Hudson, Mandy Esch, moshe levi, Joanna Kitlinska, Stella Alimperti
Glucocorticoids (GCs) are standard-of-care treatments for inflammatory and immune disorders, and their long-term use increases the risk of osteoporosis. Although GCs decrease bone functionality, their role in bone microvasculature is incompletely

Enabling Global Image Data Sharing in the Life Sciences

December 23, 2024
Author(s)
Anne L. Plant, Peter Bajcsy
Public, reference data are one of the most important foundational resources in the modern life and biomedical sciences. Over the last 40 years, the public release and availability of genomic and macromolecule structural databases accelerated discovery

Protocol for CRISPR-Cas9 genome editing of a swine cell line via electroporation

December 20, 2024
Author(s)
Patricia Kiesler, Samantha Maragh, Stella Lee, Alexis Norris, Mayumi Miller, Adam Moyer
Genome editing technology is being used in animals for a variety of purposes, including improvement of animal and public health outcomes. Characterization of genome editing reagents and anticipated genomic alterations is an essential step toward the

Trust Your Gut: Establishing Confidence in Gastrointestinal Models An Overview of the State of the Science and Contexts of Use

May 15, 2024
Author(s)
Susan Debad, David Allen, Maria Teresa Baltazar, Omari Bandele, Michaela Blaylock, Paul Brown, Maureen Bunger, Julia Co, Lynn Crosby, Amber Daniel, Steven Ferguson, Kevin Ford, Goncalo Gamboa da Costa, Kristin Gilchrist, Matthew Grogg, Maureen Gwinn, Thomas Hartung, Simon Hogan, Ye Jeong, George Kass, Elaina Kenyon, Nicole Kleinstreuer, Ville Kujala, Jaehyun Lim, Patrik Lundquist, Joanna Matheson, Shaun McCullough, Angela Melton-Celsa, Steven Musser, Ilung Oh, Oluwakemi Oyetade, Sarita Patil, Elijah Petersen, Nakissa Sadrieh, Christie Sayes, Benjamin Scruggs, Yu-Mei Tan, Bill Thelin, M. Tyler Nelson, Jose Tarazona, John Wambaugh, Jun-young Yang, Changwoo Yu, Suzanne Fitzpatrick
The webinar series and workshop titled Trust Your Gut: Establishing Confidence in Gastrointestinal Models–An Overview of the State of the Science and Contexts of Use was co-organized by NICEATM, NIEHS, FDA, EPA, CPSC, DoD, the Johns Hopkins Center for

Mechanism of Action, Potency and Efficacy: Considerations for Cell and Gene Therapies

April 1, 2024
Author(s)
Carl Simon Jr., Anne L. Plant, Catherine Zander, Erich Bozenhardt, Christina Celluzzi, David Dobnik, Melanie Grant, John Hughes, Uma Lakshmipathy, Kok-Seong Lim, Laura Montgomery, Hesham Nawar, Thiana Nebel, Linda Peltier, James Sherley, Rouzbeh Taghizadeh, Eddie Tan, Sandrine Vessillier
One of the most challenging aspects of cell and gene therapy products (CGTPs) is defining mechanism of action (MOA), potency and efficacy of the product. This perspective examines these concepts and presents helpful ways to think about them through the

Mechanical Regulation of Oral Epithelial Barrier Function

April 25, 2023
Author(s)
Eun Jin Lee, Paul Salipante, Anthony Kotula, Dana Graves, Stella Alimperti, Yoontae Kim
Epithelial cell function is modulated by mechanical forces imparted by the extracellular environment. The transmission of forces onto the cytoskeleton by modalities such as mechanical stress and matrix stiffness is necessary to address by the development

Three-dimensional, label-free cell viability measurements in tissue engineering scaffolds using optical coherence tomography

March 14, 2023
Author(s)
Greta Babakhanova, Anant Agrawal, Deepika Arora, Allison Horenberg, Jagat Budhathoki, Joy Dunkers, Joe Chalfoun, Peter Bajcsy, Carl Simon Jr.
In the field of tissue engineering, 3D scaffolds and cells are often combined to yield constructs that are used as therapeutics to repair or restore tissue function in patients. Viable cells are required to achieve the intended mechanism of action for the

Variability in genome-engineering source materials: Consider your starting point

March 10, 2023
Author(s)
Simona Patange, Sierra Miller, Samantha Maragh
The presence and impact of variability in cells as the source material for genome engineering are important to consider for the design, execution and interpretation of outcomes of a genome-engineering process. Variability may be present at the genotype and

Biglycan regulates bone development and regeneration

February 16, 2023
Author(s)
Reut Shainer, Vardit Kram, Tina Kilts, Andrew Doyle, Inbal Shainer, Carl Simon Jr., Jinyang Zeng-Brouwers, Liliana Schaefer, Marian Young
Endochondral bone development and regeneration relies on activation and proliferation of periosteum derived cells (PDCs). Biglycan (Bgn), an extracellular matrix component, is known to be expressed in bone and cartilage, however little is known about its

Fire Burn and Cauldron Bubble: What is in your Genome Editing Brew?

October 28, 2022
Author(s)
Simona Patange, Samantha Maragh
Genome editing is a rapidly evolving biotechnology with the potential to transform many sectors of industry such as agriculture, biomanufacturing, and medicine. This technology is enabled by an ever-growing portfolio of biomolecular reagents that span the

Sensor Technologies for Quality Control in Engineered Tissue Manufacturing

October 27, 2022
Author(s)
Mary Clare McCorry, Marcie Black, Chrysanthi Williams, Greta Babakhanova, Sumona Sarkar, Nathan Swami, Katherine A. Mirica, Sarah Boermeester, Abbie Underhill
The use of engineered cells, tissues, and organs has the opportunity to change the way injuries and diseases are treated. Commercialization of these groundbreaking technologies has been limited in part by the complex and costly nature of their manufacture

Micromechanical Compatibility Between Cells and Scaffolds Directs Phenotypic Transition of Stem Cells

January 28, 2022
Author(s)
Yang Song, Jiaoyue Long, Jeremiah Woodcock, Joy Dunkers, Hung Chun Lin, Douglas M. Fox, Xiaoling Liao, Yonggang Lv, Li Yang, Martin Chiang
This study experimentally substantiates the micromechanical compatibility between cell and substrate is essential for cells to achieve energetically favorable mechanotransduction that directs phenotypic transitions. The argument for this compatibility is

Gut Microbe-Targeted Choline Trimethylamine Lyase Inhibition Improves Obesity Via Rewiring of Host Circadian Rhythms

January 24, 2022
Author(s)
Rebecca Schugar, Christy Gliniak, Lucas Osborn, William Massey, Naseer Sangwan, Anthony Horak, Rakhee Banerjee, Danny Orabi, Robert Helsley, Amanda Brown, Amy Burrows, Chelsea Finney, Kevin Fung, Frederick Allen, Daniel Ferguson, Anthony Gromovsky, Chase Neumann, Kendall Cook, Amy McMillan, Jennifer Buffa, James Anderson, Margarete Mehrabian, Maryam Goudarzi, Belinda Willard, Tytus Mak, Andrew Armstrong, Garth Swanson, Ali Keshavarzian, Jose Carlos Garcia-Garcia, Zeneng Wang, Aldons Lusis, Stanley Hazen, Mark Brown
Obesity has repeatedly been linked to reorganization of the gut microbiome, yet to this point obesity therapeutics have been targeted exclusively toward the human host. Here we show that gut microbe-targeted inhibition of the trimethylamine N-oxide (TMAO)

Quantitative Determination of Cell Viability Using Absorbance Microscopy

January 19, 2022
Author(s)
Greta Babakhanova, Stephen Zimmerman, Laura Pierce, Sumona Sarkar, Nicholas Schaub, Carl Simon Jr.
Cell viability is an essential measurement for cell therapy products. One of the most common tests is Trypan blue (TB) dye exclusion where blue-stained cells are counted via imaging. Typically, live and dead cells are counted based on pixel intensities of

AbsorbanceQ: An App for Generating Absorbance Images from Brightfield Images

January 4, 2022
Author(s)
Stephen Zimmerman, Carl Simon Jr., Greta Babakhanova
The AbsorbanceQ app converts brightfield microscope images into absorbance images that can be analyzed and compared across different operators, microscopes and time. Absorbance-based measurements are comparable, which is useful when the aim is to
Displaying 1 - 25 of 80