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Displaying 276 - 300 of 802

NIST Micronutrients Measurement Quality Assurance Program (Winter 2014 Comparability Studies): Results for Round Robin LXXV Fat-Soluble Vitamins and Carotenoids in Human Serum and Round Robin 40 Ascorbic Acid in Human Serum

September 29, 2015
Author(s)
Jeanice M. Brown Thomas, David L. Duewer
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

NIST Micronutrients Measurement Quality Assurance Program Winter, Spring, and Fall 1990 Comparability Studies: Results for Round Robins XVIII, XIX, and XX Fat-Soluble Vitamins and Carotenoids in Human Serum

September 29, 2015
Author(s)
Jeanice M. Brown Thomas, David L. Duewer, Margaret C. Kline, Willie E. May, Sam A. Margolis, Neal E. Craft, Katherine E. Sharpless
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

NIST Micronutrients Measurement Quality Assurance Program Winter, Spring, and Fall 1991 Comparability Studies: Results for Round Robins XXI, XXII, and XXIII Fat-Soluble Vitamins and Carotenoids in Human Serum

September 29, 2015
Author(s)
Jeanice M. Brown Thomas, David L. Duewer, Margaret C. Kline, Katherine E. Sharpless, Willie E. May, Sam A. Margolis, Neal E. Craft
The National Institute of Standards and Technology coordinates the Micronutrients Measurement Quality Assurance Program (MMQAP) for laboratories that measure fat- and water-soluble vitamins and carotenoids in human serum and plasma. This report describes

Benchmarks for genome sequencing

September 23, 2015
Author(s)
Justin M. Zook, Marc L. Salit
In this issue, Griffith et al. present one of the most comprehensive genome-sequence data sets to date from a patient with myeloid leukemia, including sequencing of normal tissue, primary cancer, and a relapse. Their raw data from a variety of sequencing

Establishing Traceability to NIST SRM 2391c: PCR-Based DNA Profiling Standard

September 16, 2015
Author(s)
Carolyn R. Steffen, Margaret C. Kline, David L. Duewer, Peter Vallone
The NIST Standard Reference Material (SRM) 2391c: PCR-Based DNA Profiling Standard was updated in April 2015 to contain new information relevant to the forensic community. Previously, there were certified genotypes for 24 autosomal STR markers plus

Extreme expression of DNA repair protein apurinic/apyrimi-dinic endonuclease I (APE1) in human breast cancer as measured by liquid-chromatography isotope-dilution tandem mass spectrometry

September 15, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Prasad T. Reddy, Miral M. Dizdar
Apurinic/apyrimidinic endonuclease 1 (APE1) is a DNA re-pair protein and plays other important roles. Increased lev-els of APE1 in cancer have been reported. However, avail-able methods to measure APE1 levels are indirect and not quantitative. We

Extreme Expressions of DNA Repair Proteins APE1 and MTH1, in Human Breast Cancer as Measured by Liquid Chromatography and Isotope Dilution Tandem Mass Spectrometry

September 15, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Leona D. Scanlan, Alessandro Tona, Mark S. Lowenthal, Prasad T. Reddy, M Miral Dizdar, Ann-Sofie Jemth, Olga Loseva, Thomas Helleday
Accurate measurement of DNA repair proteins in cancer tissues is becoming more important due to the individual and origin based expression differences in cancer patients as well as the novel approach of using the repair enzyme inhibitors in cancer

Retention projection enables accurate calculation of liquid chromatographic retention times across labs and methods

September 3, 2015
Author(s)
Yamil Simon, Daniel Abate-Pella, Dana M. Freund, Yan Ma, Adrian Hegeman, Yan Ma, Tobias Kind, O Fiehn, Birgit Beck, Emma L. Schymanski, Corey D. Broeckling, David V. Huhman, Lloyd W. Summer, Oleg V. Krokhin, Dwight R. Stoll
Identification of small molecules by liquid chromatography-mass spectrometry (LC-MS) could be greatly improved if the chromatographic retention information could be used along with mass spectral information to narrow down the lists of potential identities

Optimizing Data Logger Setup for Vaccine Temperature Monitoring

September 1, 2015
Author(s)
Michal J. Chojnacky, Luis Chaves Santacruz, W Wyatt Miller, Gregory F. Strouse
Accidental freezing of refrigerated vaccine represents a significant public and private healthcare cost. Freeze-damaged vaccines lose their effectiveness, putting communities at risk. U.S. immunization programs have strengthened vaccine storage and

Evaluation of two mitochondrial DNA biomarkers for prostate cancer detection

August 17, 2015
Author(s)
Samantha D. Maragh, Steven P. Lund
BACKGROUND: A 3.4kb deletion (3.4kbΔ) in mitochondrial DNA (mtDNA) found in histologically normal prostate biopsy specimens has been reported to be a biomarker for the increased probability of prostate cancer. Increased mtDNA copy number is also reported

Small Molecule Inhibitors of 8-Oxoguanine DNA Glycosylase-1 (OGG1)

August 7, 2015
Author(s)
Pawel Jaruga, Erdem Coskun, M Miral Dizdar, Nathan Donley, Stephen Lloyd, Amanda McCullough
ABSTRACT The DNA base excision repair (BER) pathway, which utilizes DNA glycosylases to initiate repair of specific DNA lesions, is the major pathway for the repair of DNA damage induced by oxidation, alkylation, and deamination. Early results from

Isotopic Labeling of Proteins in Halobacterium salinarum

August 4, 2015
Author(s)
Thomas E. Cleveland, Zvi Kelman
It is often necessary to obtain isotopically labeled proteins containing 15N, 13C or 2H for NMR; and 2H for small-angle neutron scattering (SANS) or neutron diffraction studies. To achieve uniform isotopic labeling, protein expression is most commonly

Localization and Number of Au Nanoparticles in Optically Indexed Cells by FIB Tomography

August 2, 2015
Author(s)
Aric W. Sanders, Kavita M. Jeerage, Ann C. Chiaramonti Debay, Alexandra Curtin
Gold nanoparticles (GNPs) are gaining importance as therapeutic chemical delivery vehicles, medical diagnostic tools, phototherapeutic and contrast enhancement agents. GNPs are uniquely suited for these biological uses because of their chemical stability

Production, Purification and Characterization of 15N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric Measurements

July 26, 2015
Author(s)
Prasad T. Reddy, Pawel Jaruga, Bryant C. Nelson, Mark Lowenthal, Ann-Sofie Jemth, Olga Loseva, Erdem Coskun, Thomas Helleday, Miral M. Dizdar
Oxidatively induced DNA damage is caused in living organisms by a variety of damaging agents, resulting in the formation of a multiplicity of lesions, which are mutagenic and cytotoxic. Unless repaired by DNA repair mechanisms before DNA replication, DNA

Quantifying and Improving Clinical-grade Coverage and Accuracy using Augmented Exome Sequencing

July 16, 2015
Author(s)
Justin M. Zook, Anil Patwardhan, Marc L. Salit, Carlos Bustamante, Euan Ashley, Michael Snyder, John West, Richard Chen
Exome sequencing is increasingly used for the clinical evaluation of genetic disease, yet accuracy and coverage in medically interpretable parts of the genome remains under-characterized. We evaluate recently developed exome sequencing platforms in the

Best Practices for Evaluating Single Nucleotide Variant Calling Methods for Microbial Genomics

July 7, 2015
Author(s)
Nathanael D. Olson, Steven P. Lund, Rebecca Colman, Jeffery T. Foster, Jason W. Sahl, James M. Schupp, Paul Keim, Jayne B. Morrow, Marc L. Salit, Justin M. Zook
Innovations in sequencing technologies have allowed biologists to make incredible advances in understanding biological systems. As experience grows, researchers increasingly recognize that analyzing the wealth of data provided by these new sequencing

Applying Thymine Isostere 2,4-Difluoro-5-methylbenzene as an Assignment Tool and Probe of Purine-rich DNA Dynamics Using Proton NMR Spectroscopy

June 30, 2015
Author(s)
Robert G. Brinson, Jennifer T. Miller, Kahn Jason, Stuart F. Le Grice, John P. Marino
Proton assignment of NMR spectra of oligonucleotide duplexes can become difficult with increasing helical length due to cross peak overlap. Here we describe an alternative to 15N and 13C labeling that facilitates assignment via single site substitution

Addiction to MTH1 protein results in intense expression in human breast cancer tissue as measured by liquid chromatography-isotope-dilution tandem mass spectrometry

June 23, 2015
Author(s)
Erdem Coskun, Pawel Jaruga, Ann-Sofie Jemth, Olga Loseva, Leona D. Scanlan, Alessandro Tona, Mark S. Lowenthal, Thomas Helleday, M Miral Dizdar
MTH1 protein sanitizes the nucleotide pool so that modified 2'-deoxynucleoside triphosphates (dNTPs) cannot be used in DNA replication. Cancer cells require MTH1 to avoid incorporation of modified dNTPs resulting in DNA damage mutations and cell death
Displaying 276 - 300 of 802