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Search Publications

NIST Authors in Bold

Displaying 1 - 25 of 1216

Measuring the size of oil droplets in a flow cytometer using Mie Resonances

January 21, 2025
Author(s)
Richard E. Cavicchi, Dean Ripple, Joshua Welsh, Jerilyn Izac, Alexander Peterson, Aaron Goldfain, Wyatt N. Vreeland
An emulsion of silicone oil droplets in aqueous buffer produces a distinctive series of peaks or resonances in the side scatter histogram in a flow cytometer. As many as 12 peaks are observed in the violet-side scatter channel at 405 nm, with half that

Interlaboratory Measurement of Adeno-Associated Virus: Comparative Quantification of Full and Empty Capsids

December 26, 2024
Author(s)
Sean Lehman, Wyatt N. Vreeland, Jerome Jacques, Sharee Adams-Hall, Shreya Ahuja, Adnan Arnaout, Hunter Balduf, Ivan Budyak, Thomas Cleveland, Dennis Delgado, James Deng, Brandon Doyle, David L. Duewer, Carsten Elger, Jeffrey Fagan, Tim Guo, Jorge Haller, Luisa Hilgenfeld, Van Hoang, Allison Huldin, Matthew Hyatt, Sambit Kar, Sandeep Kedia, Bashkim Kokona, Amy Liu, Li Ma, Diane McCarthy, He Meng, Easton Noble, Veronika Oettle, Andrew Pla, Thomas Powers, Dean Ripple, Herbert Runnels, Raphael Ruppert, Florian Semmelmann, Christopher Sims, Saurabh Singh, Ronald Toth, Austin Vogt, Sabine Wenzel, Neal Whitaker, Zhiwen Yang, Brandon Zhuang
Recombinant adeno-associated virus (AAV) is one of the main viral vector-based gene therapy platforms. AAV is a virus consisting of a ≈25 nm diameter capsid with a ≈4.7 kb cargo capacity. The safety and efficacy depend on the correct encapsidation of the

The Application of Digital PCR as a Reference Measurement Procedure to Support the Accuracy of Quality Assurance for Infectious Disease Molecular Diagnostic Testing

December 26, 2024
Author(s)
Samreen Falak, Denise O'Sullivan, Megan Cleveland, Simon Cowen, Eloise Busby, Alison Devonshire, Esmeralda Valiente, Gerwyn Jones, Martin Kammel, Mojca Milavec, Laura Vierbaum, Ingo Schellenberg, Heinz Zeichhardt, Andreas Kummrow, Peter Vallone, Rainer Macdonald, Jim Huggett
Background Nucleic Acid Amplification Tests (NAATs) are used to diagnose many infectious diseases. They are typically sensitive and specific and can be quickly developed and adapted. It is more challenging to develop standards to ensure NAATs perform

Enabling Global Image Data Sharing in the Life Sciences

December 23, 2024
Author(s)
Anne L. Plant, Peter Bajcsy
Public, reference data are one of the most important foundational resources in the modern life and biomedical sciences. Over the last 40 years, the public release and availability of genomic and macromolecule structural databases accelerated discovery

Advancing Cell-Free Manufacturing: Challenges in Scale-up and Automation Workshop Report

November 20, 2024
Author(s)
Eugenia Romantseva, Fernanda Piorino Macruz de Oliveira, Chad Sundberg, G. Sitta Sittampalam, Elizabeth Strychalski
The workshop on Advancing Cell-free Manufacturing: Challenges in Scale-up and Automation sought to identify and prioritize actionable steps towards more reproducible, scalable, and automated cell-free systems for applications in bioengineering and

Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards

October 3, 2024
Author(s)
Tracey Schock, Christina Jones, Federico Torta, Nils Hoffmann, Bo Burla, Irina Alecu, Makoto Arita, Takeshi Bamba, Steffany A.L. Bennett, Justine BERTRAND-MICHEL, Britta Brugger, Monica Molina, Maria Dolores Camacho Munoz, Antonio Checa, Michael Chen, Michelle Cinel, Benoit COLSCH, Cristina Coman, Bebiana Da Costa Sousa, Alex Dickens, Maria Maria, Finnur Freyr Eiriksson, Hector Gallart-Ayala, Mohan Ghorasaini, Martin Giera, Xue Guan, Mark Haid, Thomas Hankemeier, Amy Harms, Marcus Horing, Michal Holcapek, Thorsten Hornemann, Chunxiu Hu, Andreas Hulsmeier, Julijana Ivanisevic, Harald Kofeler, Sin Man Lam, Jong Cheol Lee, Gerhard Liebisch, Andrea F Lopez-Clavijo, Malena Manzi, Peter Meikle, Maria Monge, Sneha Muralidharan, Anna Nicolaou, Valerie O'Donnell, Matej Oresic, Arvind Ramanathan, Daisuke Saigusa, Heidi Schwartz-Zimmermann, Guanghou Shui, Masatomo Takahashi, Margret Thorsteinsdottir, Anthony Tournadre, Hiroshi Tsugawa, Victoria Tyrrell, Grace van der Gugten, Michael Wakelam, Craig Wheelock, Denise Wolrab, Guowang Xu, John Bowden, Kim Ekroos, Robert Ahrends, Markus Wenk
In this community effort, we compared measurements between 34 laboratories from 19 countries, utilizing mixtures of authentic synthetic standards, to quantify by mass spectrometry four ceramide species in the NIST human blood plasma Standard Reference

Plug-and-play protein biosensors using aptamer-regulated in vitro transcription

September 12, 2024
Author(s)
Heonjoon Lee, Tian Xie, Byunghwa Kang, Xinjie Yu, Samuel Schaffter, Rebecca Schulman
Molecular biosensors that accurately measure protein concentrations without external equipment are critical for solving numerous problems in diagnostics and therapeutics. Modularly transducing the binding of protein antibodies, protein switches or aptamers

Quality Control in the Mass Spectrometry Proteomics Core: a Practical Primer

September 11, 2024
Author(s)
Ben Neely, Yasset Perez-Riverol, Magnus Palmblad
The past decade has seen widespread advances in quality control (QC) materials and software tools focused specifically on mass spectrometry–based proteomics, yet the rate of adoption is inconsistent. Despite the fundamental importance of QC, it typically

Multifaceted Neuroimaging Data Integration via Analysis of Subspaces

August 28, 2024
Author(s)
Andrew Ackerman, Zhengwu Zhang, Jan Hannig, Jack Prothero, J.S. Marron
Neuroimaging studies, such as the Human Connectome Project (HCP), often collect multifaceted and multi-block data to study the complex human brain. However, these data are often analyzed in a pairwise fashion, which can hinder our understanding of how

The Proteomics Standards Initiative standardized formats for spectral libraries and fragment ion peak annotations: mzSpecLib and mzPAF

August 5, 2024
Author(s)
Joshua Klein, Henry Lam, Tytus Mak, Wout Bittremieux, Yasset Perez-Riverol, Ralf Gabriels, Jim Shofstahl, Helge Hecht, Pierre-Alain Binz, Shin Kawano, Tim Van Den Bossche, Jeremy Carver, Ben Neely, Luis Mendoza, Tomi Suomi, Tine Claeys, Thomas Payne, Douwe Schulte, Zhi Sun, Nils Hoffmann, Yunping Zhu, Steffen Neumann, Andrew Jones, Nuno Bandeira, Juan Antonio Vizcaino, Eric Deutsch
Mass spectral libraries are collections of reference spectra, usually associated with specific analytes from which the spectra were generated, that are used for further downstream analysis of new spectra. There are many different formats used for encoding

Metabolomics 2023 Workshop Report: Moving Toward Consensus on Best QA/QC Practices in LC-MS-Based Untargeted Metabolomics

July 9, 2024
Author(s)
Tracey Schock, Jonathan Mosley, Warwick Dunn, Julia Kuligowski, Matthew Lewis, Maria Monge, Candice Ulmer Holland, Dajana Vuckovic, Krista Zanetti
Introduction: During the Metabolomics 2023 conference, the Metabolomics Quality Assurance and Quality Control Consortium (mQACC) presented a workshop focusing on key quality assurance and quality control (QA/QC) topic categories for LC-MS-based untargeted

Genome-wide profiling of genetic variation at tandem repeat from long reads

July 4, 2024
Author(s)
Helyaneh Jam, Justin Zook, Sara Javadzadeh, Jonghun Park, Aarushi Sehgal, Melissa Gymrek
Tandem repeats are frequent across the human genome, and variation in repeat length has been linked to a variety of traits. Recent improvements in long read sequencing technologies have the potential to greatly improve TR analysis, especially for long or

Investigation of the effect of peptide p5 targeting CDK5-p25 hyperactivity on Munc18-1(P67) regulating neuronal exocytosis using molecular simulations

July 2, 2024
Author(s)
Tejaswi Tammareddy, Walid Keyrouz, Ram Sriram, Harish Pant, Antonio Cardone, Jeffery Kluada
Munc18–1 is an SM (sec1/munc-like) family protein involved in vesicle fusion and neuronal exocytosis. Munc18–1 is known to regulate the exocytosis process by binding with closed- and open-state conformations of Syntaxin1, a protein belonging to the SNARE

Toward an International Standardisation Roadmap for Nanomedicine

June 12, 2024
Author(s)
Bryant C. Nelson, Gerrit Borchard, Luigi Calzolai, Fanny Caputo, Georges Favres, Fisicaro Fisicaro, Emeric Frejafon, Nazende Gunday-Tureli, Denis Koltsov, Caterina Minelli, Francois-Xavier Ouf, Jeremie Parot, Adriele Prina Mello, Shan Zou
The French National Measurement Institute (LNE) has initiated a series of events to identify priorities for test methods and their harmonisation that directly address regulatory needs in Nanomedicine. The workshop entitled "The International

Comprehensive Overview of Bottom-Up Proteomics using Mass Spectrometry

June 4, 2024
Author(s)
Yuming Jiang, Devasahayam Rex, Dina Schuster, Ben Neely, German Rosano, Norbert Volkmar, Amanda Momenzadeh, Trenton Peters-Clarke, Susan Egbert, Simion Kreimer, Emma Doud, Oliver Cook, Amit Yadav, Muralidharan Vanuopadath, Martin Mayta, Anna Duboff, Nicholas Riley, Robert Moritz, Jesse Meyer
Proteomics is the large scale study of protein structure and function from biological systems through protein identification and quantification. "Shotgun proteomics" or "bottom-up proteomics" is the prevailing strategy, in which proteins are hydrolyzed

Identification of Candidate Protein Biomarkers Associated with Domoic Acid Toxicosis in Cerebrospinal Fluid of California Sea Lions (Zalophus californianus)

May 28, 2024
Author(s)
Gautam Ghosh, Ben Neely, Alison Bland, Emily Whitmer, Cara Field, Padraig Duignan, Michael Janech
Since 1998, California sea lion (Zalophus californianus) stranding events associated with domoic acid toxicosis have consistently increased. Currently there are no practical non-lethal clinical tests for the diagnosis of domoic acid toxicosis (DAT) that

Standards and Metrology for Viral Vectors as Molecular Tools: Outcomes from a CCQM Workshop

May 24, 2024
Author(s)
Janathan Campbell, Neil Almond, Y Bae, Ravneet Bhuller, Andrea Briones, S-Y CHO, Megan Cleveland, Thomas Cleveland, Francis Galaway, Hua-Jun He, U Herbrand, Jim Huggett, Sarah Kempster, Ibolya Kepiro, Afifa Khan, Edward Kwee, Wilson Li, Sheng Lin-Gibson, Luise Luckau, Caterina Minelli, M Ryadnov, I Searing, Lili Wang, Alexandra Whale, Julian Braybrook
Viral vectors are agents enabling gene transfer and genome editing and have widespread utility across the healthcare and biotechnology sectors. In January 2023, the International Bureau for Weights and Measures' Consultative Committee for Amount of

Recommendations on fit-for-purpose criteria to establish a quality management for microphysiological systems (MPS) and for monitoring of their reproducibility

May 14, 2024
Author(s)
David Pamies, Jason Ekert, Marie-Gabrielle Zurich, Olivier Frey, Sophie Werner, MONICA PIERGIOVANNI, Benjamin Freedman, Adrian Keong, Darwin Reyes-Hernandez, Hendrik Erfurth, Peter Loskill, Pelin Candarlioglu, Shan Wang, Thomas Hartung, Sandra Coecke, Glyn Stacey, Marcel Leist
Cell culture technology has seen great innovations and progress in the 21st centuryClassical single-cell and monolayer models have been replaced by more complex 3D models (e.g., reaggregates, spheroids, organoids) to improve the predictivity of in vitro

Nondestructive, Longitudinal, 3D Cell Viability Assessment in a Multi-well Plate System Using Electron Paramagnetic Resonance Oxygen Imaging

May 1, 2024
Author(s)
Safa Hameed, Navin Viswakarma, Eliyas Siddiqui, Greta Babakhanova, Carl Simon Jr., Boris Epel, Mrignayani Kotecha
Cell viability is an essential measurement for cell therapy, tissue engineered medical products (TEMPs), drug development, and many other biological processes and products. These systems rely on viable, healthy, and functional cells to work as intended

Mechanism of Action, Potency and Efficacy: Considerations for Cell and Gene Therapies

April 1, 2024
Author(s)
Carl Simon Jr., Anne L. Plant, Catherine Zander, Erich Bozenhardt, Christina Celluzzi, David Dobnik, Melanie Grant, John Hughes, Uma Lakshmipathy, Kok-Seong Lim, Laura Montgomery, Hesham Nawar, Thiana Nebel, Linda Peltier, James Sherley, Rouzbeh Taghizadeh, Eddie Tan, Sandrine Vessillier
One of the most challenging aspects of cell and gene therapy products (CGTPs) is defining mechanism of action (MOA), potency and efficacy of the product. This perspective examines these concepts and presents helpful ways to think about them through the

Quantitative and Standardized Pseudovirus Neutralization Assay for COVID-19

April 1, 2024
Author(s)
Jerilyn Izac, Edward Kwee, Adolfas Gaigalas, Lili Wang
COVID-19 is a global pandemic caused by the highly infectious SARS-CoV-2 virus. Efforts to combat SARS-CoV-2 infection include mass vaccination and development of monoclonal and convalescent plasma therapeutics that require precise measurements of
Displaying 1 - 25 of 1216