U.S. flag

An official website of the United States government

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you’ve safely connected to the .gov website. Share sensitive information only on official, secure websites.

Material Measurement Laboratory

Biomolecular Measurement Division

Projects/Programs

Displaying 1 - 25 of 44
Bioman lifecycle

Biomanufacturing Initiative

Ongoing
The objective of the NIST biomanufacturing program is to support US biopharmaceutical industry delivery of high quality and low cost protein drugs around the world. The mission is fulfilled through the development of standards, measurement science, and state of the art tools that support advances in

Certification of a Standard Reference Material for Cytomegalovirus (CMV)

Ongoing
The goal of this project is to provide a SI-traceable reference material for use by calibrant and reagent manufacturers in production of their own calibrants and standards. Clinical laboratories with in-house methods would be able to use the reference material for production of their own quality

Chemical/Biochemical Microsensor Science

Ongoing
Our research is focused on the basic and applied aspects of chemical sensor science and technology in the following areas: The development and evaluation of nanoscale materials, e.g., nanowires and nanotubes, for high-performance chemical sensing of gas-phase species Novel approaches for low-cost
component 1

Consistency Analysis and Uncertainty in ‘omic Data

Ongoing
‘Omics is increasingly moving out of the laboratory and towards use in industrial and commercial applications. For instance, in biomanufacturing, there is a need for quality control when developing biotherapeutics, which will almost certainly require a machine-learning classifier to separate

Development of Proteomics-Based Methods for Protein Quantification

Ongoing
Reference measurement procedures and standard reference materials help ensure that routine clinical assays provide accurate results across different laboratories and over time. Lists of such higher-order methods are published by the Joint Committee for Traceability in Laboratory Medicine (JCTLM)

Development of Rapid Multiplex PCR Techniques

Ongoing
With the continuing development of miniaturization technologies such as microfluidic and micro-capillary devices there is a desire to reduce the overall time required to type DNA samples. Such miniature devices could be used for initial screening at a crime scene, at a border, or at airports. There

Development of Reference Methods and Reference Materials for Proteins

Ongoing
Accuracy and comparability of clinical assays is essential for timely and accurate medical interventions. However, studies have shown that large variations in results can occur among routine clinical assays. A clinical comparison among 14 commercial assay platforms revealed more than 20-fold

Digital PCR

Ongoing
With appropriately validated assays, digital polymerase chain reaction (dPCR) determines the number of DNA targets (copies) per reaction partition, without the need for a standard curve. Reaction partitions can be in the form of fixed chambers in a microfluidic device, referred to as chamber digital

DNA Biometrics

Ongoing
Our focus is on developing methods to decrease the time required to perform a DNA test and assisting with the evaluation of new prototype rapid DNA typing instrumentation. The use of rapid DNA testing as a biometric mode would impact the areas of immigration, airport and border security (Dept of

DNA Mixture Interpretation

Ongoing
Mixtures of DNA from more than one source are commonly seen in forensic evidentiary samples and are challenging to interpret. Over the past decade, our group at NIST has conducted several interlaboratory studies involving mixture interpretation with short tandem repeat (STR) markers in order to

DNA Profiling Standard Reference Materials

Ongoing
Intended impact This work was designed to provide materials that forensic laboratories being assessed under the FBI DNA Quality Assurance Standard can comply with as stated, "The laboratory shall check its DNA procedures annually or whenever substantial changes are made to the protocol(s) against an
CE and sequencing results for STR D3S1358 an allele 15 example

Forensic Applications of Next Generation Sequencing

Ongoing
Forensic DNA profiles have historically been generated by Capillary Electrophoresis (CE), which results in length-based designations reported as the number of repeats at STR (short tandem repeat) markers. For example, the marker D3S1358 contains repeats of the four nucleotides “TCTA” or “TCTG”. When
13 tubes each containing an individual glycan in SRM 3655

Glycan Standard Reference Materials

Ongoing
Now Available A glycan-based NIST Standard Reference Material ( SRM 3655 Glycans in Solution) supports the biomanufacturing and protein therapeutics communities with a set of 13 individually-bottled, pure glycoforms including those most commonly observed as N-linked glycans on therapeutic monoclonal

HDX-MS for Biopharmaceutical Analysis

Ongoing
I ntended Impact This project is designed to improve, test, and validate hydrogen/deuterium exchange mass spectrometry (HDX-MS) metrology for the determination of dynamical properties of therapeutic proteins and glycoproteins. Objectives Explore structure-function relationships between glycan
Cryogenic electron microscopy (cryo-EM) image of an LNP/RNA formulation

LNP-RNA Research Grade Test Material

Ongoing
The currently planned LNP/RNA material (designated RGTM 10240) will include a fragment of the SARS-CoV-2 genome that is approximately 4 kb in length. This fragment was previously included (in unencapsulated form) as “Fragment 1” of NIST RGTM 10169. The RNA fragment is not designed for cell-based
Fab Domain from NISTmAb

Metrologies for Protein Structure, Dynamics and Function

Ongoing
Based on its established expertise, infrastructure, and resources for structure/function studies of biological macromolecules, the program aims to impact advances in physics, chemistry, and engineering relevant to biological problems. NIST researchers also develop new technologies for application to
NIST MS Search 2

Monoclonal Antibody Mass Spectral Libraries

Ongoing
Intended Impact Modern mass spectrometers used in the field of proteomics and glycomics are capable of profiling hundreds, and even thousands of molecules in a single experiment. Each of these compounds is isolated and fragmented to form a mass spectrum. Therefore, interpretation of these mass