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Projects/Programs

Displaying 1 - 25 of 46

RM 8376 Microbial Pathogen DNA Standards for Detection and Identification

Ongoing
RM 8376 consists of 20 individual gDNA components from 19 bacterial strains and 1 human reference genome. Its purpose is to enable developers and regulators to evaluate the analytical performance of metagenomic next generation sequencing (mNGS) tools. The material is certified for chromosomal copy

Biosurveillance and Pathogen Detection

Ongoing
NIST has established partnerships with other federal agencies to develop standards and measurement solutions to support biosurveillance. These efforts are supported in part through interagency agreements with partner agencies listed below to address their standards needs. PROGRAMS Standards for

Building Measurement Confidence for Cell Characterization

Ongoing
NIST collaborates with key stakeholders in industry, other government agencies, and academia to develop measurement assurance strategies and advanced measurement capabilities for cell characterization. Data and concepts developed through workshops and laboratory programs inform the development of

Cancer Biomarker Measurements and Collaborations

Ongoing
Collaborations and Cooperative Research Agreements We welcome collaborations and can help with assay validation and development of reference materials. Below are some examples of our collaborations and ways we can develop test materials. Early Detection Research Network (EDRN): Interagency agreement

Cancer Genome in a Bottle

Ongoing
Goals: This project is an extension of the Genome in a Bottle Consortium to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of cancer genome sequencing to clinical practice and innovations in technologies. The priority of GIAB

Cell Line Authentication

Ongoing
Cell Line Authentication – The scientific community has responded to the misidentification of human cell lines with validated methods to authenticate these cells; however, there are few assays available for nonhuman cell line identification. We have developed multiplex polymerase chain reaction (PCR

Chinese hamster ovary (CHO) cell line authentication

Ongoing
Intended impact To develop a PCR based multiplex assay that can be used to authenticate Chinese hamster ovary cell lines which are important in the production of biological therapeutics. Objectives Identify tetranucleotide STR markers by searching the CHO genome using BLAST Design and optimize a

Clinical and Forensic Reference Materials

Ongoing
Below is a listing of the reference materials currently produced by the Applied Genetics group. Clinical Diagnostics SRM 2393 - CAG Repeat Length Mutation in Huntington`s Disease SRM 2365 - BK Virus DNA Quantitative Standard SRM 2366a - Cytomegalovirus DNA (Towne Δ147 BAC) for DNA SRM 2367 - JC

Combating Antibiotic Resistance

Ongoing
We are developing in vitro systems to generate controlled chemical and community environments that mimic the features of real-world that are important determinants for evolutionary rate and resistance potential. In addition, we are developing tools to isolate and analyze single bacteria along the

Comparative Mammalian Proteome Aggregator Resource (CoMPARe) Program

Ongoing
Comparative proteomics strives to gain insight into key underlying molecular changes that result in unique phenotypes across related taxa. Proteomic analysis complements comparative genomics by providing evidence of protein abundance, orthogonal to gene copy number and amount of transcript. To

Comparative Serum Proteomics Project

Ongoing
State-of-the-art biomolecular analysis is no longer limited to model organisms and is becoming routine in non-model organisms. Major drivers of this emerging bioanalytical capacity include increasing accessibility and quality of sequenced genomes as well as high-resolution fast-duty cycle mass

External RNA Controls Consortium

Completed
While early gene expression measurements with DNA microarrays were groundbreaking in their ability to reveal biological activity, the results were irreconcilable and irreproducible. Industry leaders approached the National Institute of Standards and Technology (NIST) in 2003 for help with addressing

Forensic Genetics

Ongoing
Since the late 1980s, NIST has had scientists involved in DNA testing (aka "DNA Fingerprinting"). Early concerns over measurement accuracy and poor quality control of forensic DNA tests caused the Department of Justice to call upon NIST scientists to help with standards development and technology

Genome in a Bottle

Ongoing
Consortium goals: The Genome in a Bottle Consortium is a public-private-academic consortium hosted by NIST to develop the technical infrastructure (reference standards, reference methods, and reference data) to enable translation of whole human genome sequencing to clinical practice and innovations

Genome in a Bottle Workshops

Ongoing
GIAB has held 1-2 workshops each year since being formed in 2012. These workshops include progress updates, presentations about related work, and planning for new samples and analyses. Following are links to agendas, reports, and presentations from past workshops. Workshop Reports APRIL 2020

Human Gut Microbiome Reference Material

Ongoing
The most common measurements currently being used to describe these complex microbial communities are: NGS-based metagenomics Mass spectrometry-based metabolomics In either case, no fit-for-purpose standards exist that enable researchers to compare results generated across different laboratories and

H5 Influenza Positive Controls

Ongoing
In response to the current H5N1 avian influenza outbreak, NIST has developed a research grade test material (RGTM) for PCR-based assays. This material contains 3 vials with RNA fragments for HA (Part A), NA (Part B), and MP (Part C). A NIST Reference Material is also under development. The guidance

Living Yeast Cell Reference Material (RM 8230)

Ongoing
Quantification of total and viable microbial cells is critical for applications ranging from assessment of DNA extraction efficiency in support of microbial metagenomics to characterization of antimicrobial efficacy. In addition, safe biological materials are needed to substitute for true biothreat

Microbial Genomic Measurements

Completed
The RM 8375 is a stable and homogeneous material whose intended use is whole genome sequencing quality control and proficiency testing. The genomic DNA is intended to be analyzed in the same way as any other sample a laboratory would analyze extracted DNA, such as through the use of genome assembly